2024-03-28T08:51:07Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/336552011-03-29T22:00:00Zcom_10261_80com_10261_1col_10261_333
Different Modes of Interaction in Cyanobacterial Complexes of Plastocyanin and Cytochrome f
Díaz-Moreno, Irene
Díaz-Quintana, Antonio
Rosa, Miguel A. de la
Crowley, Peter B.
Ubbink, Marcellus
Electron transfer
Photosynthesis
Plastocyanin
Cytochrome f
Cyanobacterium
Nostoc
8 páginas, 6 figuras, 1 tabla
The highly efficient electron-transfer chain in photosynthesis demonstrates a remarkable variation among organisms in the type of interactions between the soluble electron-transfer protein plastocyanin and it partner cytochrome f. The complex from the cyanobacterium Nostoc sp. PCC 7119 was studied using nuclear magnetic resonance spectroscopy and compared to that of the cyanobacterium Phormidium laminosum. In both systems, the main site of interaction on plastocyanin is the hydrophobic patch. However, the interaction in the Nostoc complex is highly dependent on electrostatics, contrary to that of Phormidium, resulting in a binding constant that is an order of magnitude larger at low ionic strength for the Nostoc complex. Studies of the mixed complexes show that these differences in interactions are mainly attributable to the surface properties of the plastocyanins.
This work was supported by grants from the Improving Human Potential Program of the European Commission (contract no. HPRNCT-
1999-00095), by the Spanish Ministry of Science and Technology (Grant BMC2003-00458), and by The Netherlands Organisation for Scientific Research (Grant 700.52.425). I.D.-M. was also the recipient of an individual fellowship from the Spanish Ministry of Education, Culture, and Sport(AP2000-2937).
Peer reviewed
2011-03-22T09:08:07Z
2011-03-22T09:08:07Z
2005-02
artículo
http://purl.org/coar/resource_type/c_6501
Biochemistry 44(9): 3176-3183 (2005)
0006-2960
http://hdl.handle.net/10261/33655
10.1021/bi047855z
1520-4995
en
http://dx.doi.org/10.1021/bi047855z
none
American Chemical Society