2024-03-29T10:42:15Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/2210472021-12-27T16:35:28Zcom_10261_131com_10261_2col_10261_384
Development of Electrospun Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Monolayers Containing Eugenol and Their Application in Multilayer Antimicrobial Food Packaging
Figueroa López, Kelly J.
Cabedo, Luis
Lagarón Cabello, José María
Torres Giner, Sergio
Ministerio de Ciencia, Innovación y Universidades (España)
European Commission
PHA
Essential oils
Multilayer
Antimicrobial activity
Electrospinning
In this research, different contents of eugenol in the 2.5–25 wt.% range were first incorporated into ultrathin fibers of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) by electrospinning and then subjected to annealing to obtain antimicrobial monolayers. The most optimal concentration of eugenol in the PHBV monolayer was 15 wt.% since it showed high electrospinnability and thermal stability and also yielded the highest bacterial reduction against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). This eugenol-containing monolayer was then selected to be applied as an interlayer between a structural layer made of a cast-extruded poly(3-hydroxybutyrate) (PHB) sheet and a commercial PHBV film as the food contact layer. The whole system was, thereafter, annealed at 160°C for 10 s to develop a novel multilayer active packaging material. The resultant multilayer showed high hydrophobicity, strong adhesion and mechanical resistance, and improved barrier properties against water vapor and limonene vapors. The antimicrobial activity of the multilayer structure was also evaluated in both open and closed systems for up to 15 days, showing significant reductions (R ≥ 1 and < 3) for the two strains of food-borne bacteria. Higher inhibition values were particularly attained against S. aureus due to the higher activity of eugenol against the cell membrane of Gram positive (G+) bacteria. The multilayer also provided the highest antimicrobial activity for the closed system, which better resembles the actual packaging and it was related to the headspace accumulation of the volatile compounds. Hence, the here-developed multilayer fully based on polyhydroxyalkanoates (PHAs) shows a great deal of potential for antimicrobial packaging applications using biodegradable materials to increase both quality and safety of food products.
This research was funded by the Spanish Ministry of Science and Innovation (MICI) through the RTI2018-097249-B-C21 program number and the H2020 EU project YPACK (reference number 773872).
Peer reviewed
2020-10-13T05:23:28Z
2020-10-13T05:23:28Z
2020-09-03
artículo
http://purl.org/coar/resource_type/c_6501
Frontiers in Nutrition 7: 140 (2020)
http://hdl.handle.net/10261/221047
10.3389/fnut.2020.00140
2296-861X
http://dx.doi.org/10.13039/501100000780
33015118
en
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-097249-B-C21
info:eu-repo/grantAgreement/EC/H2020/773872
Publisher's version
https://doi.org/10.3389/fnut.2020.00140
Sí
open
Frontiers Media