2024-03-29T09:34:16Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1904782022-10-04T10:49:56Zcom_10261_94com_10261_8col_10261_347
Methyl-end desaturases with ∆12 and ω3 regioselectivities enable the de novo PUFA biosynthesis in the cephalopod Octopus vulgaris
Garrido, Diego
Kabeya, Naoki
Hontoria, Francisco
Navarro, Juan Carlos
Reis, Diana B.
Martín, Virginia
Rodríguez, Covadonga
Almansa, E.
Monroig, Óscar
Ministerio de Economía y Competitividad (España)
CSIC - Instituto Español de Oceanografía (IEO)
University of Stirling
Garrido, Diego [0000-0002-1592-4877]
Kabeya, Naoki [0000-0002-2055-6554]
Hontoria, Francisco [0000-0003-2466-1375]
Navarro, Juan Carlos [0000-0001-6976-6686]
Monroig, Óscar [0000-0001-8712-0440]
Biosynthesis
Essential fatty acids
Methyl-end desaturases
Octopus vulgaris
The interest in understanding the capacity of aquatic invertebrates to biosynthesise omega-3 (ω3) long-chain (≥C20) polyunsaturated fatty acids (LC-PUFA) has increased in recent years. Using the common octopus Octopus vulgaris as a model species, we previously characterised a ∆5 desaturase and two elongases (i.e. Elovl2/5 and Elovl4) involved in the biosynthesis of LC-PUFA in molluscs. The aim of this study was to characterise both molecularly and functionally, two methyl-end (or ωx) desaturases that have been long regarded to be absent in most animals. O. vulgaris possess two ωx desaturase genes encoding enzymes with ∆12 and ω3 regioselectivities enabling the de novo biosynthesis of the C18 PUFA 18:2ω6 (LA, linoleic acid) and 18:3ω3 (ALA, α-linolenic acid), generally regarded as dietary essential for animals. The O. vulgaris ∆12 desaturase (“ωx2”) mediates the conversion of 18:1ω9 (oleic acid) into LA, and subsequently, the ω3 desaturase (“ωx1”) catalyses the ∆15 desaturation from LA to ALA. Additionally, the O. vulgaris ω3 desaturase has ∆17 capacity towards a variety of C20 ω6 PUFA that are converted to their ω3 PUFA products. Particularly relevant was the affinity of the ω3 desaturase towards 20:4ω6 (ARA, arachidonic acid) to produce 20:5ω3 (EPA, eicosapentaenoic acid), as supported by yeast heterologous expression, and enzymatic activity exhibited in vivo when paralarvae were incubated in the presence of [1-14C]20:4ω6. These results confirmed that several routes enabling EPA biosynthesis are operative in O. vulgaris whereas ARA and docosahexaenoic acid (DHA, 22:6ω3) should be considere
This study was funded by the Ministerio de Ciencia e Innovación (Spanish Government) under Project OCTOWELF (Ref. AGL2013-49101-C2-1-R). The authors wish to thank the Instituto Español de Oceanografía for the grant FPI 2011 “Biomarcadores de estrés y metabolismo lipídico en las primeras fases de vida del pulpo común (Octopus vulgaris)” (BOE of 3rd November 2011), as well as COST Action FA1301 (Ref. COST-STSM-ECOST-STSM-FA1301-030815-063868) by funding DG during his visit at Institute of Aquaculture, University of Stirling (Scotland, UK). Further funding was obtained through a Proyecto Intramural Especial of CSIC (201840I016) awarded to ÓM. CR is a member of the Instituto de Biotecnología de Canarias (ITB).
Peer reviewed
2019-09-11T11:22:01Z
2019-09-11T11:22:01Z
2019-08
artículo
http://purl.org/coar/resource_type/c_6501
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids 1864(8): 1134-1144 (2019)
0006-3002
http://hdl.handle.net/10261/190478
10.1016/j.bbalip.2019.04.012
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100011034
http://dx.doi.org/10.13039/501100002326
en
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-49101-C2-1-R
Postprint
http://dx.doi.org/10.1016/j.bbalip.2019.04.012
Sí
open
Elsevier