2024-03-29T05:03:53Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1901902022-10-04T12:42:19Zcom_10261_94com_10261_8col_10261_347
Dysregulation of B cell activity during proliferative kidney disease in rainbow trout
Abós, Beatriz
Estensoro, Itziar
Perdiguero, Pedro
Faber, Marc
Hu, Yehfang
Rosales, Patricia Díaz
Granja, Aitor G.
Secombes, Chris J.
Holland, Jason W.
Tafalla, Carolina
European Commission
Generalitat Valenciana
Ministry of Education (Taiwan)
Comunidad de Madrid
Perdiguero, Pedro [0000-0002-5471-9550]
Tetracapsuloides bryosalmonae
Proliferative kidney disease
Rainbow trout
B cells
Immunoglobulin T
Immunoglobulin D
Immunoglobulin M
Proliferative kidney disease (PKD) is a widespread disease caused by the endoparasite Tetracapsuloides bryosalmonae (Myxozoa: Malacosporea). Clinical disease, provoked by the proliferation of extrasporogonic parasite stages, is characterized by a chronic kidney pathology with underlying transcriptional changes indicative of altered B cell responses and dysregulated Thelper celllike activities. Despite the relevance of PKD to European and North American salmonid aquaculture, no studies, to date, have focused on further characterizing the B cell response during the course of this disease. Thus, in this work, we have studied the behavior of diverse B cell populations in rainbow trout (Oncorhynchus mykiss) naturally infected with T. bryosalmonae at different stages of preclinical and clinical disease. Our results show a clear
upregulation of all trout immunoglobulins (Igs) (IgM, IgD, and IgT) demonstrated by immunohistochemistry and Western blot analysis, suggesting the alteration of diverse B cell populations that coexist in the infected kidney. Substantial changes in IgM, IgD, and IgT repertoires were also identified throughout the course of the disease
further pointing to the involvement of the three Igs in PKD through what appear to be independently regulated mechanisms. Thus, our results provide strong evidence of the involvement of IgD in the humoral response to a specific pathogen for the first time in teleosts. Nevertheless, it was IgT, a fishspecific Ig isotype thought to be
specialized in mucosal immunity, which seemed to play a prevailing role in the kidney response to T. bryosalmonae. We found that IgT was the main Ig coating extrasporogonic parasite stages, IgT+ B cells were the main B cell subset that proliferated in the kidney with increasing kidney pathology, and IgT was the Ig for which more significant changes in repertoire were detected. Hence, although our results demonstrate a
profound dysregulation of different B cell subsets during PKD, they point to a major involvement of IgT in the immune response to the parasite. These results provide further insights into the pathology of PKD that may facilitate the future development of control strategies.
This work was supported by the European Research Council (ERC Consolidator Grant 2016 725061 TEMUBLYM) and the European Commission under the H2020 Programme (Grant H2020-634429 ParaFishControl). IE was recipient of APOSTD/2016/037 grant by the “Generalitat Valenciana” and
YH was recipient of a PhD Studentship from the Ministry of Education, Republic of China (Taiwan). JWH was supported by BBSRC grant BB/K009125/1 and SNSF grant CRSII3_147649-1. PDR was funded by grant T1-BIO-1672 from the “Comunidad de Madrid”.
Peer reviewed
2019-09-09T08:43:48Z
2019-09-09T08:43:48Z
2018-05-31
artículo
http://purl.org/coar/resource_type/c_6501
Frontiers in Immunology 9: 1203 (2018)
1664-3224
http://hdl.handle.net/10261/190190
10.3389/fimmu.2018.01203
http://dx.doi.org/10.13039/100010002
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/100012818
http://dx.doi.org/10.13039/501100003359
29904385
en
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/634429
Publisher's version
http://dx.doi.org/10.3389/fimmu.2018.01203
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open
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