2024-03-29T13:22:33Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/186032016-05-24T09:29:08Zcom_10261_91com_10261_8col_10261_344
Structure of the mexicain-E-64 complex and comparison with other cysteine proteases of the papain family
Gavira Gallardo, J. A.
González-Ramírez, L. A.
Oliver-Salvador, M. C.
Soriano-García, M.
García Ruiz, Juan Manuel
Mexicain
Cysteine proteases
E-64
Inhibitors
9 pages, 4 tables, 5 figures.
Mexicain is a 23.8 kDa cysteine protease from the tropical plant Jacaratia mexicana. It is isolated as the most abundant product after cation-exchange chromatography of the mix of proteases extracted from the latex of the fruit. The purified enzyme inhibited with E-64 [N-(3-carboxyoxirane-2-carbonyl)-leucyl-amino(4-guanido)butane] was crystallized by sitting-drop vapour diffusion and the structure was solved by molecular replacement at 2.1 Å resolution and refined to an R factor of 17.7% (Rfree = 23.8%). The enzyme belongs to the [alpha]+[beta] class of proteins and the structure shows the typical papain-like fold composed of two domains, the [alpha]-helix-rich (L) domain and the [beta]-barrel-like (R) domain, separated by a groove containing the active site formed by residues Cys25 and His159, one from each domain. The four monomers in the asymmetric unit show one E-64 molecule covalently bound to Cys25 in the active site and differences have been found in the placement of E-64 in each monomer.
This work was supported by the Spanish Ministry of Science and the Technology Project ESP2003-04759, the Andalucia
Regional Government Project RNM1344 and by the Mexican National Polytechnic Institute (IPN) Projects CGPI 20021341 and 20030510. This paper is a result of the Factoría de Cristalización funded by the program onsolider-Ingenio 2010. JAG was supported by the Andalusian Regional Government, Spain. We also would like to thank Dr Ana Ámara-Artiga for her helpful comments and revision of the manuscript.
Peer reviewed
2009-11-13T09:02:58Z
2009-11-13T09:02:58Z
2007-05
artículo
http://purl.org/coar/resource_type/c_6501
Acta Crystallographica Section D 63(5): 555-563 (2007)
0108-2701
http://hdl.handle.net/10261/18603
10.1107/S0907444907005616
en
http://dx.doi.org/10.1107/S0907444907005616
none
13824 bytes
application/vnd.ms-excel
International Union of Crystallography