2024-03-28T19:33:24Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1846232021-12-27T16:41:45Zcom_10261_86com_10261_1col_10261_339
Identification and expression of the 11b-steroid hydroxylase from Cochliobolus lunatus in Corynebacterium glutamicum
Felpeto-Santero, Carmen
Galán, Beatriz
Luengo, José M.
Fernández-Cañón, José Manuel
Del Cerro, Carlos
Medrano, Francisco Javier
García, José Luis
Ministerio de Ciencia e Innovación (España)
Galán, Beatriz [0000-0002-2596-6034]
Luengo, José M. [0000-0002-4984-6256]
Fernández-Cañón, José Manuel [0000-0002-8092-2181]
Del Cerro, Carlos [0000-0003-1693-3895]
Medrano, Francisco Javier [0000-0002-8185-9751]
García, José Luis [0000-0002-9238-2485]
Hydroxylation of steroids has acquired special relevance for the pharmaceutical industries. Particularly, the 11β-hydroxylation of steroids is a reaction of biotechnological importance currently carried out at industrial scale by the fungus Cochliobolus lunatus. In this work, we have identified the genes encoding the cytochrome CYP103168 and the reductase CPR64795 of C. lunatus responsible for the 11β-hydroxylase activity in this fungus, which is the key step for the preparative synthesis of cortisol in industry. A recombinant Corynebacterium glutamicum strain harbouring a plasmid expressing both genes forming a synthetic bacterial operon was able to 11β-hydroxylate several steroids as substrates. This is a new example to show that the industrial strain C. glutamicum can be used as a suitable chassis to perform steroid biotransformation expressing eukaryotic cytochromes.
This work was supported by grants from the Ministry of Science and Innovation (BFU2006-15214-C03-01, BFU2009-11545-C03-03) and Ministry of Economy and Competitiveness (BIO2012-39695-C02-01).
Peer reviewed
2019-06-21T11:48:23Z
2019-06-21T11:48:23Z
2019-06-14
artículo
http://purl.org/coar/resource_type/c_6501
Microb Biotechnol 12(5): 856–868 (2019)
http://hdl.handle.net/10261/184623
10.1111/1751-7915.13428
1751-7915
http://dx.doi.org/10.13039/501100004837
31197939
en
Publisher's version
https://doi.org/10.1111/1751-7915.13428
Sí
open
John Wiley & Sons