2024-03-28T14:58:08Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/180892019-02-14T10:46:17Zcom_10261_72com_10261_6col_10261_325
Phenolic Compounds and Related Enzymes Are Not Rate-Limiting in Browning Development of Fresh-Cut Potatoes
Cantos Villar, Emma
Tudela, Juan Antonio
Gil Muñoz, M.ª Isabel
Espín de Gea, Juan Carlos
Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Ministerio de Ciencia y Tecnología (España)
Ministerio de Educación, Cultura y Deporte (España)
Enzymatic browning
Fresh-cut potato
Isoelectric focusing
Minimal processing
Phenylalanine ammonia-lyase
PAL
Peroxidase
POD
Polyphenol oxidase
PPO
9 pages, 7 figures, 2 tables.
The effect of minimal processing on polyphenol oxidase (PPO), peroxidase (POD), phenylalanine ammonia-lyase (PAL), and phenolic compounds was studied in five potato cultivars (Agria, Cara, Liseta, Monalisa, and Spunta). Minimal processing caused an overall increase in PPO, POD, and PAL activities. The isoform pattern of PPO was the same for all of the cultivars before and after processing. No latent PPO was detected. The isoperoxidase pattern was approximately the same among cultivars. An increase in POD activity was related to the specific induction of an acidic isoperoxidase. PAL showed an induction pattern characterized by the presence of a maximum peak of activity after 4 days of processing for all of the cultivars. The sequence of browning susceptibility of potato cultivars was as follows: Monalisa > Spunta > Liseta > Cara > Agria. Browning development was only partially correlated to PAL activity (only during the first 4 days after wounding). However, this correlation could not explain the above sequence of browning susceptibility. Minimal processing caused an increase of chlorogenic acid, whereas tyrosine content remained unchanged. In summary, no significant correlation was found between either rate or degree of browning and any other biochemical and physiological attribute investigated (PPO, POD, hydrogen peroxide, ascorbic acid content, and initial phenolics content as well as total and individual phenolics accumulation).
This work has been partially supported by a grant from CICYT, Spain, Projects AGL2000-
0452-P4-03 and AGL2001-1269. E.C. is holder of a grant from the “Movilidad de Investigadores y Tecnólogos” program under the action “Mit-Becas; Modalidad F2”. J.A.T. has a fellowship from Ministerio de Educación, Cultura y Deporte (Spain), Reference AP2001-2493.
Peer reviewed
2009-10-27T11:30:11Z
2009-10-27T11:30:11Z
2002-04-09
artículo
http://purl.org/coar/resource_type/c_6501
Journal of Agricultural and Food Chemistry 50(10): 3015-3023 (2002)
0021-8561
http://hdl.handle.net/10261/18089
10.1021/jf0116350
1520-5118
http://dx.doi.org/10.13039/501100007273
http://dx.doi.org/10.13039/501100006280
http://dx.doi.org/10.13039/501100003176
en
http://dx.doi.org/10.1021/jf0116350
none
259768 bytes
application/pdf
American Chemical Society