2024-03-28T18:09:04Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1709922018-10-12T00:56:14Zcom_10261_14com_10261_8col_10261_267
Cortisol modulates vasotocinergic and isotocinergic pathways in the gilthead sea bream
Cádiz, Laura
Román-Padilla, Javier
Gozdowska, Magdalena
Kulczykowska, Ewa
Martínez-Rodríguez, Gonzalo
Mancera, Juan Miguel
Martos-Sitcha, Juan Antonio
Ministerio de Ciencia e Innovación (España)
Ministry of Science and Higher Education (Poland)
Cortisol
Isotocin
Receptors
Sparus aurata
Stress
Vasotocin
In the present study, we assessed the responses of the vasotocinergic and isotocinergic systems to chronic stress induced by cortisol administration in the gilthead sea bream (Sparus aurata). Pituitary and plasma arginine vasotocin (AVT) and isotocin (IT) levels, as well as hypothalamic pro-vasotocin (pro-VT) and pro-isotocin (pro-IT) mRNA expression levels, were analysed. In addition, the mRNA levels of three receptors, AVTR type V1a2, AVTR type V2 and ITR, were analysed in several target organs associated with the following physiological processes: (i) integration and control (hypothalamus), (ii) metabolism and its control (liver and hypothalamus), (iii) osmoregulation (gills) and (iv) stress response (head kidney). Specimens were injected intraperitoneally with slow-release implants (5 μL g−1 body mass) containing coconut oil alone (control group) or with cortisol (50 μg g−1 body mass; cortisol group). Both AVT and IT synthesis and release were correlated with plasma cortisol values, suggesting a potential interaction between both hormonal systems and cortisol administration. Our results suggest that the activation of hepatic metabolism as well as the hypothalamic control of metabolic processes provide the energy necessary to overcome stress, which could be partly mediated by AVTRs and ITR. Upregulation of branchial AVT and IT receptor expression following cortisol treatment suggests an involvement of the vasotocinergic and isotocinergic systems in the regulation of ion channels/transporters during stressful situations. Finally, changes in AVT and IT receptor mRNA expression in the head kidney suggest these nonapeptides participate in feedback mechanisms that regulate the synthesis/release of cortisol. Our results indicate a relationship between cortisol and both the vasotocinergic and isotocinergic systems during simulated chronic stress in S. aurata.
This study was funded by project AGL2010-14876 from the Ministry of Science and Innovation to J.M.M. (Spain) and by project 498/N-HISZP-JPR/2009/0 to E.K. (Poland).
Peer reviewed
2018-10-11T12:41:05Z
2018-10-11T12:41:05Z
2015
artículo
http://purl.org/coar/resource_type/c_6501
Journal of Experimental Biology 218: 316-325 (2015)
0022-0949
http://hdl.handle.net/10261/170992
10.1242/jeb.113944
1477-9145
http://dx.doi.org/10.13039/501100004837
en
Publisher's version
https://doi.org/10.1242/jeb.113944
Sí
open
Company of Biologists