2024-03-28T13:17:11Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1670282018-08-01T09:49:12Zcom_10261_74com_10261_6col_10261_327
Low‐gluten, nontransgenic wheat engineered with CRISPR/Cas9
Sánchez‐León, Susana
Gil Humanes, Javier
Ozuna, Carmen
Giménez, María J.
Sousa Martín, Carolina
Voytas, Daniel F.
Barro Losada, Francisco
Ministerio de Economía y Competitividad (España)
European Commission
Fundación Alfonso Martín Escudero
Coeliac disease
a -gliadins
CRISPR/Cas9
Coeliac disease is an autoimmune disorder triggered in genetically predisposed individuals by the ingestion of gluten proteins from wheat, barley and rye. The α‐gliadin gene family of wheat contains four highly stimulatory peptides, of which the 33‐mer is the main immunodominant peptide in patients with coeliac. We designed two sgRNAs to target a conserved region adjacent to the coding sequence for the 33‐mer in the α‐gliadin genes. Twenty‐one mutant lines were generated, all showing strong reduction in α‐gliadins. Up to 35 different genes were mutated in one of the lines of the 45 different genes identified in the wild type, while immunoreactivity was reduced by 85%. Transgene‐free lines were identified, and no off‐target mutations have been detected in any of the potential targets. The low‐gluten, transgene‐free wheat lines described here could be used to produce low‐gluten foodstuff and serve as source material to introgress this trait into elite wheat varieties.
The Spanish Ministry of Economy and Competitiveness (Projects AGL2013‐48946‐C3‐1‐R and AGL2016‐80566‐P) and the European Regional Development Fund (FEDER) supported this work. Javier Gil‐Humanes acknowledges the Fundacion Alfonso Martín Escudero for its funding support.
Peer reviewed
2018-06-26T11:54:55Z
2018-06-26T11:54:55Z
2018-04
artículo
http://purl.org/coar/resource_type/c_6501
Plant Biotechnology Journal 16(4): 902-910 (2018)
1467-7644
http://hdl.handle.net/10261/167028
1467-7652
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/100008052
http://dx.doi.org/10.13039/501100003329
en
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013‐48946‐C3‐1‐R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016‐80566‐P
Publisher's version
http://dx.doi.org/10.1111/pbi.12837
Sí
open
Wiley-Blackwell