2024-03-29T00:36:45Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1442082020-05-20T12:44:33Zcom_10261_79com_10261_1com_10261_108com_10261_8col_10261_332col_10261_361
Expression of heat shock and other stress response proteins in ticks and cultured tick cells in response to Anaplasma spp. infection and heat shock
Villar, Margarita
Ayllón, Nieves
Busby, Ann T.
Galindo, Ruth C.
Blouin, Edmour F.
Kocan, Katherine M.
Bonzón-Kulichenko, Elena
Zivkovic, Zorica
Almazán, Consuelo
Torina, Alessandra
Vázquez, Jesús
Fuente, José de la
Ministerio de Ciencia e Innovación (España)
European Commission
Consejo Superior de Investigaciones Científicas (España)
Sitlington Endowment Funds
Ministerio de Educación y Ciencia (España)
Ticks are ectoparasites of animals and humans that serve as vectors of Anaplasma and other pathogens that affect humans and animals worldwide. Ticks and the pathogens that they transmit have coevolved molecular interactions involving genetic traits of both the tick and the pathogen that mediate their development and survival. In this paper, the expression of heat shock proteins (HSPs) and other stress response proteins (SRPs) was characterized in ticks and cultured tick cells by proteomics and transcriptomics analyses in response to Anaplasma spp. infection and heat shock. The results of these studies demonstrated that the stress response was activated in ticks and cultured tick cells after Anaplasma spp. infection and heat shock. However, in the natural vector-pathogen relationship, HSPs and other SRPs were not strongly activated, which likely resulted from tick-pathogen coevolution. These results also demonstrated pathogen- and tick-specific differences in the expression of HSPs and other SRPs in ticks and cultured tick cells infected with Anaplasma spp. and suggested the existence of post-transcriptional mechanisms induced by Anaplasma spp. to control tick response to infection. These results illustrated the complexity of the stress response in ticks and suggested a function for the HSPs and other SRPs during Anaplasma spp. infection.
This research was supported by the Ministerio de Ciencia e Innovacion, Spain (Project no. BFU2008-01244/BMC), the CSIC intramural Project no. PA1002451 to J. Fuente, and
the Walter R. Sitlington Endowed Chair for Food Animal Research to K. M. Kocan M. Villar and R. C. Galindo were funded by the JAE-DOC program (CSIC-FSE) and MEC,
Spain, respectively.
Peer Reviewed
2017-02-17T12:29:31Z
2017-02-17T12:29:31Z
2010
2017-02-17T12:29:31Z
artículo
http://purl.org/coar/resource_type/c_6501
doi: 10.1155/2010/657261
issn: 2090-2166
e-issn: 2090-2174
International Journal of Proteomics: 657261 (2010)
http://hdl.handle.net/10261/144208
10.1155/2010/657261
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003339
22084679
Publisher's version
https://doi.org/10.1155/2010/657261
Sí
open
Hindawi Publishing Corporation