2024-03-29T15:02:42Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1433902021-12-27T16:02:28Zcom_10261_125com_10261_2col_10261_378
Impact of prematurity and perinatal antibiotics on the developing intestinal microbiota: A functional inference study
Arboleya, Silvia
Sánchez García, Borja
Solís, Gonzalo
Fernández, Nuria
Suárez, Marta
Hernández-Barranco, Ana María
Margolles Barros, Abelardo
González de los Reyes-Gavilán, Clara
Gueimonde Fernández, Miguel
European Commission
Ministerio de Economía y Competitividad (España)
Principado de Asturias
Consejo Superior de Investigaciones Científicas (España)
Antibiotics
Infants
Preterm
Intestinal microbiota
Microbiome
[Background] The microbial colonization of the neonatal gut provides a critical stimulus for
normal maturation and development. This process of early microbiota establishment, known to be
affected by several factors, constitutes an important determinant for later health.
[Methods] We studied
the establishment of the microbiota in preterm and full-term infants and the impact of perinatal
antibiotics upon this process in premature babies. To this end, 16S rRNA gene sequence-based
microbiota assessment was performed at phylum level and functional inference analyses were
conducted. Moreover, the levels of the main intestinal microbial metabolites, the short-chain fatty
acids (SCFA) acetate, propionate and butyrate, were measured by Gas-Chromatography Flame
ionization/Mass spectrometry detection.
[Results] Prematurity affects microbiota composition at
phylum level, leading to increases of Proteobacteria and reduction of other intestinal microorganisms.
Perinatal antibiotic use further affected the microbiota of the preterm infant. These changes involved
a concomitant alteration in the levels of intestinal SCFA. Moreover, functional inference analyses
allowed for identifying metabolic pathways potentially affected by prematurity and perinatal
antibiotics use.
[Conclusion] A deficiency or delay in the establishment of normal microbiota function
seems to be present in preterm infants. Perinatal antibiotic use, such as intrapartum prophylaxis,
affected the early life microbiota establishment in preterm newborns, which may have consequences
for later health.
This work was founded by the EU Joint Programming Initiative–A Healthy Diet for a
Healthy Life (JPI HDHL, http://www.healthydietforhealthylife.eu/) and the Spanish Ministry of Economy
and Competitiveness (MINECO) (Project EarlyMicroHealth). Borja Sánchez was the recipient of a Ramón y
Cajal Postdoctoral contract (RYC-2012-10052) from MINECO. The Grant GRUPIN14-043 from “Plan Regional de
Investigación del Principado de Asturias” is also acknowledged.
We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI).
Peer Reviewed
2017-02-03T12:37:50Z
2017-02-03T12:37:50Z
2016-04-29
2017-02-03T12:37:50Z
artículo
http://purl.org/coar/resource_type/c_6501
issn: 1422-0067
International Journal of Molecular Sciences 17: 649 (2016)
http://hdl.handle.net/10261/143390
10.3390/ijms17050649
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/100011941
27136545
Publisher's version
https://doi.org/10.3390/ijms17050649
Sí
open
Molecular Diversity Preservation International