2024-03-28T22:29:41Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1427662021-12-27T15:41:26Zcom_10261_125com_10261_2col_10261_378
Different metabolic features 1 of Bacteroides fragilis growing in the presence of glucose and exopolysaccharides of bifidobacteria
Rios-Covián, David
Sánchez García, Borja
Salazar, Nuria
Martínez Álvarez, Noelia
Redruello, Begoña
Gueimonde Fernández, Miguel
González de los Reyes-Gavilán, Clara
Ministerio de Economía y Competitividad (España)
European Commission
Principado de Asturias
Bifidobacterium
Probiotics
Metabolism
Glucose
Exopolysaccharides
Bacteroides fragilis
Bacteroides is among the most abundant microorganism inhabiting the human intestine. They are saccharolytic bacteria able to use dietary or host-derived glycans as energy sources. Some Bacteroides fragilis strains contribute to the maturation of the immune system but it is also an opportunistic pathogen. The intestine is the habitat of most Bifidobacterium species, some of whose strains are considered probiotics. Bifidobacteria can synthesize exopolysaccharides (EPS), which are complex carbohydrates that may be available in the intestinal environment. We studied the metabolism of B. fragilis when an EPS preparation from bifidobacteria was added to the growth medium compared to its behavior with added glucose. 2D-DIGE coupled with the identification by MALDI-TOF/TOF evidenced proteins that were differentially produced when EPS was added. The results were supported by RT qPCR gene expression analysis. The intracellular and extracellular pattern of certain amino acids, the redox balance and the a-glucosidase activity were differently affected in EPS with respect to glucose. These results allowed us to hypothesize that three general main events, namely the activation of amino acids catabolism, enhancement of the transketolase reaction from the pentose-phosphate cycle, and activation of the succinate-propionate pathway, promote a shift of bacterial metabolism rendering more reducing power and optimizing the energetic yield in the form of ATP when Bacteroides grow with added EPS. Our results expand the knowledge about the capacity of B. fragilis for adapting to complex carbohydrates and amino acids present in the intestinal environment. © 2015 Rios-covian, Sanchez, Salazar, Martinez, Redruello, Gueimonde and De los Reyes-Gavilan.
This work was financed by projects AGL2010-16525 and AGL2013-43770-R from Plan Nacional/Plan Estatal de I+D+I (Spanish Ministry of Economy and Competitiveness, MINECO). The activity of Probiotics and Prebiotics Group is being partly supported through the Grant GRUPIN14-043 from Plan Regional de Investigación del Principado de Asturias. Both, national and regional grants received cofounding from European Union FEDER funds. DR-C was the recipient of predoctoral FPI fellowship whereas BS enjoys a Ramon and Cajal contract from MINECO. NS benefits from a Clarin post-doctoral contract (Marie Curie European CoFund Program) cofinanced by Plan Regional de Investigación del Principado de Asturias, Spain. We acknowledge the excellent technical assistance of Lidia Alaez, whose technician contract was partially supported by the project AGL2010-16525 and by Plan Regional de Investigación del Principado de Asturias, through the grant COF 13-020.
Peer Reviewed
2017-01-19T13:25:18Z
2017-01-19T13:25:18Z
2015-08-18
2017-01-19T13:25:19Z
artículo
http://purl.org/coar/resource_type/c_6501
issn: 1664-302X
Frontiers in Microbiology 6 (2015)
http://hdl.handle.net/10261/142766
10.3389/fmicb.2015.00825
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/100011941
26347720
Publisher's version
https://doi.org/10.3389/fmicb.2015.00825
Sí
open
Frontiers Media