2024-03-29T09:26:41Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1427552021-11-22T13:02:29Zcom_10261_125com_10261_2col_10261_378
A single mutation in the gene responsible for the mucoid phenotype of Bifidobacterium animalis subsp. lactis confers surface and functional characteristics
Hidalgo-Cantabrana, Claudio
Sánchez García, Borja
Martínez Álvarez, Noelia
Ruas-Madiedo, Patricia
Margolles Barros, Abelardo
European Commission
Centro de Investigación Biomédica en Red Enfermedades Hepáticas y Digestivas (España)
Ministerio de Ciencia e Innovación (España)
Exopolysaccharides (EPS) are extracellular carbohydrate polymers synthesized by a large variety of bacteria. Their physiological functions have been extensively studied, but many of their roles have not yet been elucidated. We have sequenced the genomes of two isogenic strains of Bifidobacterium animalis subsp. lactis that differ in their EPS-producing phenotype. The original strain displays a nonmucoid appearance, and the mutant derived thereof has acquired a mucoid phenotype. The sequence analysis of their genomes revealed a nonsynonymous mutation in the gene Balat_1410, putatively involved in the elongation of the EPS chain. By comparing a strain from which this gene had been deleted with strains containing the wild-type and mutated genes, we were able to show that each strain displays different cell surface characteristics. The mucoid EPS synthesized by the strain harboring the mutation in Balat_1410 provided higher resistance to gastrointestinal conditions and increased the capability for adhesion to human enterocytes. In addition, the cytokine profiles of human peripheral blood mononuclear cells and ex vivo colon tissues suggest that the mucoid strain could have higher anti-inflammatory activity. Our findings provide relevant data on the function of Balat_1410 and reveal that the mucoid phenotype is able to alter some of the most relevant functional properties of the cells.
This work was financed by the Spanish Ministry of Science and Innovation (MICINN) and FEDER European Union funds through project AGL2012-33278, by Fondo de Investigación Sanitaria grant PI12/00263, and by CIBERehd. C. Hidalgo-Cantabrana acknowledges his FPI fellowship, and B. Sánchez acknowledges his postdoctoral contract Ramón y Cajal corresponding to MICINN.
Peer Reviewed
2017-01-19T12:44:33Z
2017-01-19T12:44:33Z
2015-09-11
2017-01-19T12:44:33Z
artículo
http://purl.org/coar/resource_type/c_6501
issn: 0099-2240
Applied and Environmental Microbiology 81(23): 7960-7968 (2015)
http://hdl.handle.net/10261/142755
10.1128/AEM.02095-15
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100004837
26362981
Postprint
https://doi.org/10.1128/AEM.02095-15
Sí
none
American Society for Microbiology