2024-03-28T19:17:34Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1277602021-12-27T16:30:41Zcom_10261_41com_10261_1com_10261_79col_10261_294col_10261_332
Highly sensitive molecular diagnosis of prostate cancer using surplus material washed off from biopsy needles
Bermudo, Raquel
Abia, David
Mozos, A.
García-Cruz, E.
Alcaraz, A.
Ortiz, Ángel R.
Thomson, Timothy M.
Fernández, Pedro L.
Ministerio de Educación (España)
Xarxa de Bancs de Tumors de Catalunya
Fundación Ramón Areces
Ministerio de Ciencia e Innovación (España)
Fundació La Marató de TV3
Ministerio de Sanidad y Consumo (España)
Instituto de Salud Carlos III
Xarxa de Bancs de Tumors de Catalunya
Linear discriminant analysis
Gene signature
Needle biopsy
Prostate cancer
Diagnosis
Real-time RT–PCR
INTRODUCTION: Currently, final diagnosis of prostate cancer (PCa) is based on histopathological analysis of needle biopsies, but this process often bears uncertainties due to small sample size, tumour focality and pathologist’s subjective assessment.
METHODS: Prostate cancer diagnostic signatures were generated by applying linear discriminant analysis to microarray and real-time RT–PCR (qRT–PCR) data from normal and tumoural prostate tissue samples. Additionally, after removal of biopsy tissues, material washed off from transrectal biopsy needles was used for molecular profiling and discriminant analysis.
RESULTS: Linear discriminant analysis applied to microarray data for a set of 318 genes differentially expressed between non-tumoural and tumoural prostate samples produced 26 gene signatures, which classified the 84 samples used with 100% accuracy. To identify signatures potentially useful for the diagnosis of prostate biopsies, surplus material washed off from routine biopsy needles from 53 patients was used to generate qRT–PCR data for a subset of 11 genes. This analysis identified a six-gene signature that correctly assigned the biopsies as benign or tumoural in 92.6% of the cases, with 88.8% sensitivity and 96.1% specificity.
CONCLUSION: Surplus material from prostate needle biopsies can be used for minimal-size gene signature analysis for sensitive and accurate discrimination between non-tumoural and tumoural prostates, without interference with current diagnostic procedures. This approach could be a useful adjunct to current procedures in PCa diagnosis.
British Journal of Cancer (2011) 105, 1600–1607. doi:10.1038/bjc.2011.435 www.bjcancer.com
Published online 18 October 2011
& 2011 Cancer Research UK
Ministerio de Ciencia e Innovacion (PI080274), Fundación Marato TV3, Ministerio de Educacio´n (GEN2001-4856- C13, GEN2001-4865-C13-10 and SAF2005-05109), Ministerio de Sanidad (PI020231), Red Temática de Cáncer of the Instituto Carlos III (ISCIII-RETIC RD06/0020), Xarxa de Bancs de Tumors de Catalunya-ICO (XBTC) and Fundación Ramón Areces.
Peer Reviewed
2016-01-19T09:21:25Z
2016-01-19T09:21:25Z
2011
2016-01-19T09:21:25Z
artículo
http://purl.org/coar/resource_type/c_6501
doi: 10.1038/bjc.2011.435
issn: 0007-0920
British Journal of Cancer 105: 1600- 1607 (2011)
http://hdl.handle.net/10261/127760
10.1038/bjc.2011.435
http://dx.doi.org/10.13039/100008054
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/100008666
http://dx.doi.org/10.13039/501100004587
22009027
Publisher's version
http://dx.doi.org710.1038/bjc.2011.435
Sí
open
Nature Publishing Group