2024-03-28T15:43:18Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/125622022-11-24T11:57:52Zcom_10261_5063com_10261_5col_10261_5066
Evaluation of eye and skin irritation of arginine-derivative surfactants using different in vitro endpoints as alternatives to the in vivo assays
MartÃnez, V.
Corsini, E
Mitjans, Montserrat
Pinazo Gassol, Aurora
Vinardell, M. Pilar
Arginine-derivative surfactant
Skin irritation
Eye irritation
Cytotoxicity
Cytokine
IL-1alpha
9 pages, 5 figures, 4 tables.
Arginine-derivative surfactants constitute a novel class of surfactants, which can be regarded as an alternative to conventional surfactants. Prior to human exposure, it is necessary to assess their irritation potential. The classical in vivo evaluation of the irritancy potential via the Draize test has been extensively criticized. In that regard, a great number of in vitro alternatives have been developed. Erythrocytes were chosen as the target cells for eye irritation assessment and hemolysis and hemoglobin denaturation were selected as appropriate endpoints. For skin irritancy assessment, the keratinocyte cell line NCTC 2544 was used and different in vitro endpoints were measured: two cytotoxicity assays (NRU and MTT) and the synthesis of the proinflammatory cytokine IL-1alpha. The eye and skin Draize tests were also performed for comparative purposes. The results point out that, according to in vivo and in vitro assays, the new arginine-derivative surfactants have lower eye and skin irritation potential than the synthetic surfactant SDS. Furthermore, in vitro methods were also able to detect differences in irritancy among the new surfactants not noticeable by the Draize tests, indicating that in vitro methods can be more sensitive than the in vivo test, offering the opportunity to detect subtle differences in irritancy.
This research was supported by the Project PPQ-2003-01834 from MCTE (Spain). Veronica Martinez holds a doctoral grant from the University of Barcelona (Spain).
Peer reviewed
2009-04-27T08:13:39Z
2009-04-27T08:13:39Z
2006-07-15
artÃculo
http://purl.org/coar/resource_type/c_6501
Toxicology Letters 164 (3):259-267 (2006)
0378-4274
http://hdl.handle.net/10261/12562
10.1016/j.toxlet.2006.01.005
en
http://dx.doi.org/10.1016/j.toxlet.2006.01.005
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19968 bytes
application/msword
Elsevier