2024-03-29T13:20:45Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1030482022-03-25T10:51:27Zcom_10261_47com_10261_8col_10261_300
Identification of Atlantic cod (Gadus morhua), ling (Molva molva), and Alaska pollock (Gadus chalcogrammus) by PCR-ELISA using duplex PCR
Taboada, Ledicia
Sánchez, Ana Cristina
Velasco, Amaya
Santaclara, Francisco J.
Pérez Martín, Ricardo Isaac
González Sotelo, Carmen
Gadus chalcogrammus
Gadus morhua
Molva molva
Species identification
PCR−ELISA
8 páginas, 5 figuras, 5 tablas
Species-specific PCR–ELISA assays for the identification of Atlantic cod (Gadus morhua), Alaska pollock (Gadus chalcogrammus), and ling (Molva molva) in food products have been developed. The method, comprising a set of primers common to the first two species, a set of primers for M. molva, and a probe for each species, was designed using ND4 and cytochrome b genes as molecular markers. The sensitivity and selectivity were then determined for each assay. These assays were afterward used to analyze DNA extracted from commercial fish products. The presence of the target species was successfully detected in all analyzed samples, demonstrating the applicability of this method to the analysis of food products.
The work was supported by the projects
“
GENTRASEA:
Genetic traceability of
fish products. Rapid methods with DNA
hybridization probes
”
and
“
LABELFISH: Atlantic network on
genetic control of
fi
sh and seafood labelling and traceability
”
.
The Spanish Ministry of Science and Innovation is gratefully
acknowledged for the doctoral fellowship to L.T
Peer reviewed
2014-10-08T11:32:15Z
2014-10-08T11:32:15Z
2014
artículo
http://purl.org/coar/resource_type/c_6501
Journal of Agricultural and Food Chemistry 62(24): 5699-5706 (2014)
0021-8561
http://hdl.handle.net/10261/103048
10.1021/jf500173j
1520-5118
en
http://dx.doi.org/10.1021/jf500173j
Sí
open
American Chemical Society