2024-03-29T15:07:58Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1002652018-08-29T11:39:22Zcom_10261_31891com_10261_2col_10261_31892
Release of multifunctional peptides by gastrointestinal digestion of sea cucumber (Isostichopus badionotus)
Gómez-Ruiz, José Ángel
Hernández-Ledesma, Blanca
Consejo Superior de Investigaciones Científicas (España)
European Commission
Simulated gastrointestinal digestion
Isostichopus badionotus
Sea cucumber
Biological activities
Bioactive peptides
Sea cucumber is a benthic marine organism distributed worldwide and used as food in several Asian countries. The species Isostichopus badionotus is captured intensively off the Yucatan Peninsula, Mexico. Boiled I. badionotus was subjected to in vitro simulated gastrointestinal digestion using pepsin and a pepsin-Corolase PP® mixture. ACE-inhibitory and radical scavenging activities, iron reducing capacity and cytotoxic effects against colorectal cancer cells were evaluated in the hydrolysates and their ultrafiltered fractions. ACE-inhibitory activity was potent in fractions containing peptides <3000Da, an effect augmented with combined action of gastric (pepsin) and intestinal (Corolase PP®) enzymes (IC50=0.038±0.004mg/mL). Antioxidant activity was exerted by peptides with low and high molecular weights, depending on hydrolysis method. This is the first report of cytotoxic capacity against colorectal HT-29 cells in peptides from sea cucumber. Sea cucumber hydrolysates and ultrafiltered fractions are potential ingredients for development of functional foods. © 2013 Elsevier Ltd.
The research reported here forms part of a project financed by the FOMIX-Yucatan (Code M0023. No. 108373). B.H.L. thanks the Spanish National Research Council (CSIC) for her Ramón y Cajal post-doctoral research contract.
Peer Reviewed
2014-07-21T11:41:34Z
2014-07-21T11:41:34Z
2013
2014-07-21T11:41:34Z
artículo
http://purl.org/coar/resource_type/c_6501
doi: 10.1016/j.jff.2013.01.036
issn: 1756-4646
Journal of Functional Foods 5(2): 869-877 (2013)
http://hdl.handle.net/10261/100265
10.1016/j.jff.2013.01.036
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/501100000780
none
Elsevier