2024-03-29T01:19:24Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/361892020-11-12T14:39:25Zcom_10261_38com_10261_5com_10261_86com_10261_1com_10261_34col_10261_291col_10261_339col_10261_287
Barrientos, Africa G.
Martínez de la Fuente, Jesús
Jiménez, Marta
Solís, Dolores
Cañada, F. Javier
Martín-Lomas, Manuel
Penadés, Soledad
2011-05-27T12:38:22Z
2011-05-27T12:38:22Z
2009-08-17
Carbohydrate Research 344(12): 1474-1478 (2009)
0008-6215
http://hdl.handle.net/10261/36189
10.1016/j.carres.2009.04.029
Glyconanoparticles (GNPs) are water-soluble carbohydrate-functionalized gold nanoclusters with a promising potential to serve as versatile tools in studies ranging from basic chemical glycobiology to clinical applications. In this paper we evaluate the influence of ligand density and presentation on the recognition by protein receptors by examining the interaction of lactose-functionalized GNPs with two different galactose-specific carbohydrate-binding proteins: an enzyme, Escherichia coli β-galactosidase, and a lectin, Viscum album agglutinin. The results suggest that the proper selection of ligand densities and spacers in GNP functionalization is an important requisite to match the topological requirements of the target receptor while escaping glycosidase degradation.
eng
closedAccess
Gold glyconanoparticles
Glycosidases
Lectins
Multivalent presentation
Molecular recognition
Modulating glycosidase degradation and lectin recognition of gold glyconanoparticles
artículo