2024-03-28T13:49:34Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/314132021-06-11T11:35:08Zcom_10261_10252com_10261_3com_10261_36com_10261_4com_10261_25com_10261_1col_10261_10253col_10261_289col_10261_278
Treviño, Juan
Calle Martín, Ana
Rodríguez Frade, José Miguel
Mellado, Mario
Lechuga, Laura M.
2011-01-21T10:09:42Z
2011-01-21T10:09:42Z
2009-05
Clinica Chimica Acta 403(1-2): 56-62 (2009)
0009-8981
http://hdl.handle.net/10261/31413
10.1016/j.cca.2009.01.015
http://dx.doi.org/10.13039/100008666
http://dx.doi.org/10.13039/501100006373
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/100004319
Background: Direct determination of four pituitary peptide hormones: human thyroid stimulating hormone (hTSH), growth hormone (hGH), follicle stimulating hormone (hFSH), and luteinizing hormone (hLH) has been carried out using a portable surface plasmon resonance (SPR) immunosensor.
Methods: A commercial SPR biosensor was employed. The immobilization of the hormones was optimized and monoclonal antibodies were selected in order to obtain the best sensor performance. Assay parameters as running buffer and regeneration solution composition or antibody concentration were adjusted to achieve a sensitive analyte detection.
Results: The performance of the assays was assessed in buffer solution, serum and urine, showing sensitivity in the range from 1 to 6 ng/mL. The covalent attachment of the hormones ensured the stability of the SPR signal through repeated use in up to 100 consecutive assay cycles. Mean intra- and inter-day coefficients of variation were all < 7%, while batch-assay variability using different sensor surfaces was < 5%.
Conclusions: Taking account both the excellent reutilization performance and the outstanding reproducibility, this SPR immunoassay method turns on a highly reliable tool for endocrine monitoring in laboratory and point-of-care (POC) settings.
eng
closedAccess
SPR
Immunosensor
hTSH
hGH
hFSH
hLH
Surface plasmon resonance immunoassay analysis of pituitary hormones in urine and serum samples
artículo