2024-03-28T13:43:13Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/274822017-07-05T09:44:22Zcom_10261_50com_10261_8col_10261_303
Verdoy, Dolores
Lucas, M. Mercedes
Manrique, Esteban
Covarrubias, A. A.
Felipe, Mª Rosario de
Pueyo, José Javier
2010-09-07T12:06:59Z
2010-09-07T12:06:59Z
2004
Plant, Cell and Environment 27: 757-767 (2004)
0140-7791
http://hdl.handle.net/10261/27482
Nodulated bean plants were exposed to mild salt stress or
water deficit in such a way that the nodule’s nitrogen-fixing
activity was reduced to about 25–30% that of controls.
Water-deprived plants showed a slight decrease in the
weight of the aerial part, whereas the photosynthetic
parameters were not significantly affected. In contrast, saltstressed
plants displayed a reversible decrease in the quantum
yield of photosystem II photochemistry. Five waterdeficit
responsive cDNA clones encoding one lipid transfer
protein, two late-embryogenesis abundant (LEA) proteins
and two proline-rich proteins (PRPs) showed different
organ-specific expression patterns depending on the kind
of stress applied. PRPs and one LEA protein, PvLEA-18,
exhibited the highest expression in nodules. Anti-PvLEA-
18 antibodies were used to immunolocalize the protein in
the nodule. PvLEA-18 was localized in the cytoplasm and
nucleus of nodule cortex cells, and preferentially in cells of
the vascular bundles, showing enhanced accumulation
under water deficit. To our knowledge, this is the first time
that a LEA protein has been identified in legume nodules.
eng
closedAccess
Late-embryogenesis abundant proteins
Legumes
Lipid transfer proteins
Nitrogen fixation
Nodule
Proline-rich proteins
Differential organ-specific response to salt stress and water deficit in nodulated bean (Phaseolus vulgaris)
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