2024-03-28T20:44:07Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1911312022-03-29T11:12:41Zcom_10261_94com_10261_8col_10261_473
Rakus, K.
Ronsmans, M.
Forlenza, Maria
Piazzon de Haro, María Carla
Wiegertjes, Geert F.
Boutier, M.
Jazowiecka-Rakus, J.
Gatherer, D.
Athanasiadis, A.
Farnir, F.
Davison, Andrew J.
Boudinot, P.
Michiels, T.
Vanderplasschen, A.
2019-09-20T08:58:51Z
2019-09-20T08:58:51Z
2017-09-04
18th International Conference on Diseases of Fish and Shellfish (2017)
http://hdl.handle.net/10261/191131
When infected by pathogens, endotherms and ectotherms can both increase their body
temperature to limit the infection. Ectotherms do so by moving to warmer places, hence the
term ¿behavioral fever¿. We studied the expression of behavioral fever by common carp
infected by cyprinid herpesvirus 3 (CyHV-3) using multi-chamber tanks encompassing a
24°C-32°C gradient. We showed that carp maintained at 24°C all died from the infection,
whereas those housed in multichamber tanks all survived as a consequence of their transient
migration to the warmest compartment. As the expression of behavioral fever occurred only
at an advanced stage of the disease, we hypothesized that the virus might delay this
phenomenon in order to promote its replication. This hypothesis was proved correct, and the
delay mechanism was found to rely on the expression of a soluble viral decoy receptor for
Tnf¿ encoded by CyHV-3 ORF12. This conclusion relied on three complementary
observations: (i) a CyHV3 ORF12 deleted recombinant induced an early onset of behavioral
fever in comparison to wild-type CyHV-3; (ii) ORF12 expression product binds and
neutralizes carp Tnf¿; and (iii) injection of anti-Tnf¿ neutralizing antibodies suppressed
behavioral fever, and decreased fish survival in response to infection. This study provides a
unique example of how viruses have evolved to alter host behavior to increase fitness. It
demonstrates that behavioral fever in ectotherms and fever in endotherms are evolutionarily
and functionally related through common cytokine mediators that originated more than 400
million years ago. Finally, this study stresses the importance of the environment in the hostpathogen-
environment triad.
eng
closedAccess
Conserved fever pathways across vertebrates: A herpesvirus delays fish behavioral fever through expression of a decoy Tnf-alpha receptor
comunicación de congreso