2024-03-29T00:41:32Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1813982022-12-21T08:33:35Zcom_10261_36com_10261_4col_10261_289
Malvar, Óscar
Ruz Martínez, José Jaime
Kosaka, Priscila M.
Domínguez, Carmen M.
Gil-Santos, Eduardo
Calleja, Montserrat
Tamayo de Miguel, Francisco Javier
2019-05-14T10:44:37Z
2019-05-14T10:44:37Z
2016-11-11
Nature Communications 7: 13452 (2016)
http://hdl.handle.net/10261/181398
10.1038/ncomms13452
2041-1723
http://dx.doi.org/10.13039/501100000781
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003329
27834379
The identification of species is a fundamental problem in analytical chemistry and biology. Mass spectrometers identify species by their molecular mass with extremely high sensitivity (<10−24 g). However, its application is usually limited to light analytes (<10−19 g). Here we demonstrate that by using nanomechanical resonators, heavier analytes can be identified by their mass and stiffness. The method is demonstrated with spherical gold nanoparticles and whole intact E. coli bacteria delivered by electrospray ionization to microcantilever resonators placed in low vacuum at 0.1 torr. We develop a theoretical procedure for obtaining the mass, position and stiffness of the analytes arriving the resonator from the adsorption-induced eigenfrequency jumps. These results demonstrate the enormous potential of this technology for identification of large biological complexes near their native conformation, a goal that is beyond the capabilities of conventional mass spectrometers.
eng
http://creativecommons.org/licenses/by/4.0/
openAccess
Mass and stiffness spectrometry of nanoparticles and whole intact bacteria by multimode nanomechanical resonators
artículo