2024-03-28T08:50:29Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1742442021-11-22T13:07:34Zcom_10261_41com_10261_1col_10261_294
Hernández-Vega, Amayra
Marsal, María
Pouille, Philippe‐Alexandre
Tosi, Sébastien
Colombelli, Julien
Luque, Tomás
Navajas, Daniel
Pagonabarraga, Ignacio
Martín‐Blanco, Enrique
2019-01-17T10:57:33Z
2019-01-17T10:57:33Z
2017-01-04
EMBO Journal 36(1): 25-41 (2017)
0261-4189
http://hdl.handle.net/10261/174244
10.15252/embj.201694264
1460-2075
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100002809
27834222
The principles underlying the biomechanics of morphogenesis are largely unknown. Epiboly is an essential embryonic event in which three tissues coordinate to direct the expansion of the blastoderm. How and where forces are generated during epiboly, and how these are globally coupled remains elusive. Here we developed a method, hydrodynamic regression (HR), to infer 3D pressure fields, mechanical power, and cortical surface tension profiles. HR is based on velocity measurements retrieved from 2D+T microscopy and their hydrodynamic modeling. We applied HR to identify biomechanically active structures and changes in cortex local tension during epiboly in zebrafish. Based on our results, we propose a novel physical description for epiboly, where tissue movements are directed by a polarized gradient of cortical tension. We found that this gradient relies on local contractile forces at the cortex, differences in elastic properties between cortex components and the passive transmission of forces within the yolk cell. All in all, our work identifies a novel way to physically regulate concerted cellular movements that might be instrumental for the mechanical control of many morphogenetic processes.
eng
closedAccess
Epiboly
Hydrodynamics
Mechanics
Morphogenesis
Zebrafish
Polarized cortical tension drives zebrafish epiboly movements
artículo