2024-03-29T12:38:16Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1624542021-07-28T08:00:29Zcom_10261_101com_10261_5col_10261_354
Gascón Pérez, Victoria
Carucci, Cristina
Jiménez, Mayra B.
Blanco Martín, Rosa María
Sánchez Sánchez, Manuel
Magner, Edmond
2018-03-19T12:44:29Z
2018-03-19T12:44:29Z
2017-04-07
ChemCatChem 9(7): 1182-1186 (2017)
1867-3880
http://hdl.handle.net/10261/162454
10.1002/cctc.201601342
1867-3899
http://dx.doi.org/10.13039/501100002081
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100001602
The use of a metal–organic framework (MOF) as a support for the in situ immobilization of enzymes was explored. The MOF support, a Basolite F300‐like material, was prepared from FeCl3 and the tridentate linker trimesic acid. Immobilization of alcohol dehydrogenase, lipase, and glucose oxidase was performed in situ under mild conditions (aqueous solution, neutral pH, and at room temperature) in a rapid and facile manner with retention of activity for at least 1 week. The catalytic activities of lipase and glucose oxidase were similar to the activities of the free enzymes; with alcohol dehydrogenase, there was a substantial decrease in activity on immobilization that may arise from diffusion limitations. The approach demonstrates that a MOF material, prepared from cheap and commercially available materials, can be successively utilized to prepare stable and catalytically active biocatalysts in a rapid and facile manner.
eng
openAccess
Rapid In Situ Immobilization of Enzymes in Metal–Organic Framework Supports under Mild Conditions
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