2024-03-29T05:44:16Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1509232020-11-12T14:39:23Zcom_10261_86com_10261_1col_10261_339
Espaillat, Akbar
Forsmo, Oskar
El Biari, Khouzaima
Björk, Rafael
Lemaitre, B.
Trygg, Johan
Cañada, F. Javier
Pedro, Miguel Ángel de
Cava, Felipe
2017-06-05T12:41:52Z
2017-06-05T12:41:52Z
2016-06-23
J. Am. Chem. Soc.138 (29)9193–9204 (2016)
1554-8929
http://hdl.handle.net/10261/150923
10.1021/jacs.6b04430
1554-8937
http://dx.doi.org/10.13039/501100004063
http://dx.doi.org/10.13039/501100003329
27337563
Peptidoglycan is a fundamental structure for most bacteria. It contributes to the cell morphology and provides cell wall integrity against environmental insults. While several studies have reported a significant degree of variability in the chemical composition and organization of peptidoglycan in the domain Bacteria, the real diversity of this polymer is far from fully explored. This work exploits rapid ultraperformance liquid chromatography and multivariate data analysis to uncover peptidoglycan chemical diversity in the Class
Alphaproteobacteria, a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles. Indeed, chemometric analyses revealed novel peptidoglycan structures conserved in Acetobacteria: amidation at the α-(L)-carboxyl of meso-diaminopimelic acid and the presence of muropeptides cross-linked by (1−3)L-Ala-D-(meso)-diaminopimelate cross-links. Both structures are growth-controlled modifications that influence sensitivity to Type VI secretion system peptidoglycan endopeptidases and recognition by the Drosophila innate immune system, suggesting relevant roles in the environmental adaptability of these bacteria. Collectively our findings demonstrate the discriminative power of chemometric tools on large cell wall-chromatographic data sets to discover novel peptidoglycan structural properties in bacteria.
eng
openAccess
Chemometric analysis of bacterial peptidoglycan reveals atypical modifications which empower the cell wall against predatory enzymes and fly innate immunity
artículo