2024-03-29T08:25:15Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/783802021-06-21T06:43:36Zcom_10261_125com_10261_2col_10261_378
DIGITAL.CSIC
author
Martín, M. Cruz
author
Martínez Álvarez, Noelia
author
Río Lagar, Beatriz del
author
Ladero Losada, Víctor Manuel
author
Álvarez González, Miguel Ángel
funder
Fundación para el Fomento en Asturias de la Investigación Científica Aplicada y la Tecnología
2013-06-20T12:49:24Z
2013-06-20T12:49:24Z
2010-03
Journal of Dairy Science 93(3): 860-867 (2010)
http://hdl.handle.net/10261/78380
10.3168/jds.2009-2481
http://dx.doi.org/10.13039/501100008430
The presence of lactose-fermenting Enterobacteriaceae and coliforms is routinely assessed to determine the hygienic quality of water and foods, particularly dairy products. This paper reports the use of lacZ-specific primers in an SYBR green I-based real-time PCR method for the easy and rapid detection of coliforms in dairy products. A large number of bacterial species were assayed to establish the specificity of the method. The sensitivity of the method was assessed using artificially contaminated cheeses. The limit of detection was 1 coliform cell in cheese samples enriched for 8. h in a culture medium. The entire procedure, including sample processing, enrichment, DNA extraction, and real-time PCR amplification, can be completed within 10 to 12. h, making it a single-day assay. © 2010 American Dairy Science Association.
eng
closedAccess
A novel real-time polymerase chain reaction-based method for the detection and quantification of lactose-fermenting Enterobacteriaceae in the dairy and other food industries
artículo
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URL
https://digital.csic.es/bitstream/10261/78380/1/accesoRestringido.pdf
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accesoRestringido.pdf.txt