2024-03-29T01:32:48Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/783792016-02-17T19:44:59Zcom_10261_125com_10261_2col_10261_378
DIGITAL.CSIC
author
Sánchez García, Borja
author
López, Patricia
author
González Rodríguez, Irene
author
Suárez, Ana
author
Margolles Barros, Abelardo
author
Urdaci, María C.
funder
Ministerio de Ciencia e Innovación (España)
2013-06-20T12:36:49Z
2013-06-20T12:36:49Z
2011-05
FEMS Microbiology Letters 318(2): 101-107 (2011)
http://hdl.handle.net/10261/78379
10.1111/j.1574-6968.2011.02244.x
http://dx.doi.org/10.13039/501100004837
Bacillus cereus CH is a probiotic strain used in human nutrition whose adhesion to mucin is dependent on its surface-associated flagellin. Flagellins from the surface of several probiotic Bacillus strains were efficiently extracted with 5M LiCl and identified by peptide fingerprinting. Based on the proteomic analysis, cloning of the gene coding for the flagellin of B. cereus CH was performed in the lactococcal vector pNZ8110 under the control of a nisin-inducible promoter. The resulting strain, Lactococcus lactis CH, produced a surface-associated flagellin after 6h of induction with nisin. The recombinant Lactococcus strain adhered strongly to mucin-coated polystyrene plates, whilst inhibiting competitively the adhesion of the pathogens Escherichia coli LMG2092 and Salmonella enterica ssp. enterica LMG15860 to the same molecule. Strain CH could be used in further experimentation for the characterization of the molecular mechanism of action of this probiotic B. cereus CH flagellin. © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd.
eng
closedAccess
A flagellin-producing Lactococcusstrain: Interactions with mucin and enteropathogens
artículo
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URL
https://digital.csic.es/bitstream/10261/78379/1/accesoRestringido.pdf
File
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accesoRestringido.pdf
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https://digital.csic.es/bitstream/10261/78379/5/accesoRestringido.pdf.txt
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accesoRestringido.pdf.txt