2024-03-29T09:15:00Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/742242020-10-20T10:55:01Zcom_10261_86com_10261_1col_10261_339
DIGITAL.CSIC
author
Fernández-López, Raul
author
Machón, Cristina
author
Longshaw, Christopher M.
author
Martín, Steve
author
Molin, Soren.
author
Zechner, Ellen L.
author
Espinosa, Manuel
author
Lanka, Erich
author
Cruz, Fernando de la
2013-04-12T09:31:31Z
2013-04-12T09:31:31Z
2005
Microbiology 151(11):3517-3526(2005)
http://hdl.handle.net/10261/74224
10.1099/mic.0.28216-0
This report describes a high-throughput assay to identify substances that reduce the frequency of conjugation in Gram-negative bacteria. Bacterial conjugation is largely responsible for the spread of multiple antibiotic resistances in human pathogens. Conjugation inhibitors may provide a means to control the spread of antibiotic resistance. An automated conjugation assay was developed that used plasmid R388 and a laboratory strain of Escherichia coli as a model system, and bioluminescence as a reporter for conjugation activity. Frequencies of conjugation could be measured continuously in real time by the amount of light produced, and thus the effects of inhibitory compounds could be determined quantitatively. A control assay, run in parallel, allowed elimination of compounds affecting cell growth, plasmid stability or gene expression. The automated conjugation assay was used to screen a database of more than 12 000 microbial extracts known to contain a wide variety of bioactive compounds (the NatChem library). The initial hit rate was 1.4 %. From these, 48 extracts containing active compounds and representing a variety of organisms and extraction conditions were subjected to fractionation (24 fractions per extract). The 52 most active fractions were subjected to a secondary analysis to determine the range of plasmid inhibition. Plasmids R388, R1 and RP4 were used as representatives of a variety of plasmid transfer systems. Only one fraction (of complex composition) affected transfer of all three plasmids, while four other fractions were active against two of them. Two separate compounds were identified from these fractions: linoleic acid and dehydrocrepenynic acid. Downstream analysis showed that the chemical class of unsaturated fatty acids act as true inhibitors of conjugation. © 2005 SGM.
eng
closedAccess
Unsaturated fatty acids are inhibitors of bacterial conjugation
artículo
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URL
https://digital.csic.es/bitstream/10261/74224/1/accesoRestringido.pdf
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accesoRestringido.pdf
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https://digital.csic.es/bitstream/10261/74224/5/accesoRestringido.pdf.txt
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accesoRestringido.pdf.txt