2024-03-29T15:40:09Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/535112020-11-12T14:39:24Zcom_10261_86com_10261_1col_10261_339
DIGITAL.CSIC
author
Muñoz, F. Javier
author
Pérez, José
author
Rumbero, Ángel
author
Santos, José Ignacio
author
Cañada, F. Javier
author
André, Sabine
author
Gabius, Hans-Joachim
author
Jiménez-Barbero, Jesús
author
Sinisterra, José V.
author
Hernáiz, María J.
2012-07-18T10:55:58Z
2012-07-18T10:55:58Z
2009-04
Bioconjugate Chemistry 20(4):673-682(2009)
1043-1802
http://hdl.handle.net/10261/53511
10.1021/bc800350q
1520-4812
Suitable glycan derivatives will find immediate application in the study of their interactions. Here, we present an efficient synthetic strategy to introduce a fluorescent tag functionalized with an amino group into a model disaccharide structure (lactose). This strategy led to the maintenance of bioactivity, checked by the study of the interaction of this bioconjugate with a plant lectin (mistletoe lectin 1) by NMR spectroscopy, computational docking, and surface plasmon resonance (SPR). To demonstrate the versatility of this approach, we immobilized the new glycan derivatives on different surfaces, and a comparative analysis is presented and can be successfully used for biomimetic carbohydrate−protein interaction studies on the SPR biochip
eng
closedAccess
Glycan tagging to produce bioactive ligands for a surface plasmon resonance study via immobilization on different surfaces
artículo
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URL
https://digital.csic.es/bitstream/10261/53511/1/restringido.pdf
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restringido.pdf
URL
https://digital.csic.es/bitstream/10261/53511/4/restringido.pdf.txt
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restringido.pdf.txt