2024-03-19T01:02:30Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/400962021-06-10T12:26:04Zcom_10261_79com_10261_1col_10261_332
DIGITAL.CSIC
author
Nuez, Beatriz
author
Rojo, Fernando
author
Salas, Margarita
funder
National Institutes of Health (US)
funder
Ministerio de Economía y Competitividad (España)
funder
Fundación Ramón Areces
funder
Ministerio de Educación y Ciencia (España)
2011-09-27T10:34:13Z
2011-09-27T10:34:13Z
1992-12-01
Proceedings of the National Academy of Sciences of the USA 89 (23) 11401-11405 (1992)
1091-6490
http://hdl.handle.net/10261/40096
http://dx.doi.org/10.13039/100000002http://dx.doi.org/10.13039/501100003329http://dx.doi.org/10.13039/100008054
Transcription from the late promoter, PA3, of Bacillus subtilis phage phi 29 is activated by the viral regulatory protein p4. A kinetic analysis of the activation process has revealed that the role of protein p4 is to stabilize the binding of RNA polymerase to the promoter as a closed complex without significantly affecting further steps of the initiation process. Electrophoretic band-shift assays performed with a DNA fragment spanning only the protein p4 binding site showed that RNA polymerase could efficiently retard the complex formed by protein p4 bound to the DNA. Similarly, when a DNA fragment containing only the RNA polymerase-binding region of PA3 was used, p4 greatly stimulated the binding of RNA polymerase to the DNA. These results strongly suggest that p4 and RNA polymerase contact each other at the PA3 promoter. In the light of current knowledge of the p4 activation mechanism, we propose that direct contacts between the two proteins participate in the activation process.
eng
openAccess
Phage phi 29 regulatory protein p4 stabilizes the binding of the RNA polymerase to the late promoter in a process involving direct protein-protein contacts
artículo
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URL
https://digital.csic.es/bitstream/10261/40096/1/PNAS-1992-Nuez-11401-5.pdf
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https://digital.csic.es/bitstream/10261/40096/4/PNAS-1992-Nuez-11401-5.pdf.txt
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PNAS-1992-Nuez-11401-5.pdf.txt