2024-03-28T10:09:19Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/392562018-10-17T08:28:31Zcom_10261_79com_10261_1col_10261_332
DIGITAL.CSIC
author
Zaballos, Ángel
author
Lázaro, José M.
author
Méndez Cormán, Enrique
author
Mellado, Rafael P.
author
Salas, Margarita
funder
National Institutes of Health (US)
funder
Ministerio de Economía y Competitividad (España)
funder
Instituto de Salud Carlos III
funder
Fundación Ramón Areces
2011-09-05T10:31:56Z
2011-09-05T10:31:56Z
1989-11-30
Gene 83(2): 33-40 (1989)
0378-1119
http://hdl.handle.net/10261/39256
10.1016/0378-1119(89)90104-2
http://dx.doi.org/10.13039/100000002http://dx.doi.org/10.13039/501100003329http://dx.doi.org/10.13039/100008054http://dx.doi.org/10.13039/501100004587
A series of internal deletions of gene 3, coding for the phage φ29 DNA terminal protein, have been constructed and characterized. In addition, a substitution mutant in the sequence corresponding to amino acids (aa) 49–51 was obtained. The priming activity of the substitution mutant protein, m the formation of the protein p3-dAMP initiation complex, was drastically reduced suggesting that some of the aa present at position 49–51 are essential for p3 function. Deletions of 8 to 33 aa, from aa residue 48 towards the N terminus of the substitution mutant, further decreased the priming activity of the protein. The activity of deletion mutants lacking 15 or 21 aa from residue 57 towards the C terminus, and also containing a point mutation at position 56, was greatly reduced, and no activity was seen when 24 aa were lacking.
eng
closedAccess
Recombinant DNA
Phage lambda PL promoter
Protein p3-dAMP complex
Effects of internal deletions on the priming activity of the phage ø29 terminal protein
artículo
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