2024-03-28T16:02:11Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/329482021-12-28T16:32:18Zcom_10261_118com_10261_6col_10261_371
DIGITAL.CSIC
author
Crespo, Helena
author
Reina, Ramsés
author
Glaria, Idoia
author
Ramírez, Hugo
author
Andrés, Ximena de
author
Jáuregui, Paula
author
Luján, Lluís
author
Martínez-Pomares, Luisa
author
Amorena Zabalza, Beatriz
author
Andrés, Damián F. de
2011-03-02T11:08:07Z
2011-03-02T11:08:07Z
2011-02-07
Veterinary Research 42: 28-37 (2011)
0928-4249
http://hdl.handle.net/10261/32948
10.1186/1297-9716-42-28
21314911
This study aims to characterize the mannose receptor (MR) gene in sheep and its role in ovine visna/maedi virus (VMV) infection. The deduced amino acid sequence of ovine MR was compatible with a transmembrane protein having a cysteine-rich ricin-type amino-terminal region, a fibronectin type II repeat, eight tandem C-type lectin carbohydrate-recognition domains (CRD), a transmembrane region, and a cytoplasmic carboxy-terminal tail. The ovine and bovine MR sequences were closer to each other compared to human or swine MR. Concanavalin A (ConA) inhibited VMV productive infection, which was restored by mannan totally in ovine skin fibroblasts (OSF) and partially in blood monocyte-derived macrophages (BMDM), suggesting the involvement of mannosylated residues of the VMV ENV protein in the process. ConA impaired also syncytium formation in OSF transfected with an ENV-encoding pN3-plasmid. MR transcripts were found in two common SRLV targets, BMDM and synovial membrane (GSM) cells, but not in OSF. Viral infection of BMDM and especially GSM cells was inhibited by mannan, strongly suggesting that in these cells the MR is an important route of infection involving VMV Env mannosylated residues. Thus, at least three patterns of viral entry into SRLV-target cells can be proposed, involving mainly MR in GSM cells (target in SRLV-induced arthritis), MR in addition to an alternative route in BMDM (target in SRLV infections), and an alternative route excluding MR in OSF (target in cell culture). Different routes of SRLV infection may thus coexist related to the involvement of MR differential expression.
eng
openAccess
Identification of the ovine mannose receptor and its possible role in Visna/Maedi virus infection
artículo
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https://digital.csic.es/bitstream/10261/32948/1/1297-9716-42-28.pdf
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1297-9716-42-28.pdf.txt