2024-03-19T09:38:18Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1834832020-12-11T08:58:09Zcom_10261_59com_10261_6col_10261_312
DIGITAL.CSIC
author
Baena, Irene
author
Pérez-Mendoza, Daniel
author
Sauviac, L.
author
Francesch, K.
author
Martín, Marta
author
Rivilla, Rafael
author
Bonilla, Ildefonso
author
Bruand, C.
author
Sanjuán, Juan
author
Lloret, Javier
funder
Junta de Andalucía
funder
Ministerio de Economía y Competitividad (España)
funder
European Commission
2019-06-06T09:34:26Z
2019-06-06T09:34:26Z
2019
Environmental Microbiology (2019)
http://hdl.handle.net/10261/183483
10.1111/1462-2920.14624
http://dx.doi.org/10.13039/501100000780http://dx.doi.org/10.13039/501100003329http://dx.doi.org/10.13039/501100011011
Sinorhizobium meliloti synthesizes a linear mixed-linkage (1 → 3)(1 → 4)-β-d-glucan (ML β-glucan, MLG) in response to high levels of cyclic diguanylate (c-di-GMP). Two proteins BgsA and BgsB are required for MLG synthesis, BgsA being the glucan synthase which is activated upon c-di-GMP binding to its C-terminal domain. Here we report that the product of bgrR (SMb20447) is a diguanylate cyclase (DGC) that provides c-di-GMP for the synthesis of MLG by BgsA. bgrR is the first gene of a hexacistronic bgrRSTUWV operon, likely encoding a partner-switching regulatory network where BgrR is the final target. Using different approaches, we have determined that the products of genes bgrU (containing a putative PP2C serine phosphatase domain) and bgrW (with predicted kinase effector domain), modulate the phosphorylation status and the activity of the STAS domain protein BgrV. We propose that unphosphorylated BgrV inhibits BgrR DGC activity, perhaps through direct protein–protein interactions as established for other partner switchers. A bgrRSTUWV operon coexists with MLG structural bgsBA genes in many rhizobial genomes but is also present in some MLG non-producers, suggesting a role of this partner-switching system in other processes besides MLG biosynthesis.
eng
openAccess
A partner-switching system controls activation of mixed-linkage β-glucan synthesis by c-di-GMP in Sinorhizobium meliloti
artículo
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URL
https://digital.csic.es/bitstream/10261/183483/1/2019_Baena_EM_Pre.pdf
File
MD5
15d214c5a6141ea46b1d6b9301d07e94
4952200
application/pdf
2019_Baena_EM_Pre.pdf