2024-03-29T07:10:35Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1276562018-08-02T11:47:45Zcom_10261_74com_10261_6col_10261_327
DIGITAL.CSIC
author
Cabo, Sandra
author
Ferreira, Luciana
author
Carvalho, Ana
author
Martins-Lopes, Paula
author
Martín, Antonio
author
Lima Brito, José
funder
European Commission
funder
Quadro de Referência Estratégico Nacional (Portugal)
funder
Fundação para a Ciência e a Tecnologia (Portugal)
2016-01-15T12:12:46Z
2016-01-15T12:12:46Z
2014-04-15
Journal of Applied Genetics 55(3): 307- 312 (2014)
http://hdl.handle.net/10261/127656
10.1007/s13353-014-0211-3
http://dx.doi.org/10.13039/501100000780http://dx.doi.org/10.13039/501100001871
Hexaploid tritordeum (HchHchAABB; 2n = 42) results from the cross between Hordeum chilense (HchHch; 2n = 14) and cultivated durum wheat (Triticum turgidum ssp. durum (AABB; 2n = 28). Morphologically, tritordeum resembles the wheat parent, showing promise for agriculture and wheat breeding. Start Codon Targeted (SCoT) polymorphism is a recently developed technique that generates gene-targeted markers. Thus, we considered it interesting to evaluate its potential for the DNA fingerprinting of newly synthesized hexaploid tritordeums and their respective parents. In this study, 60 SCoT primers were tested, and 18 and 19 of them revealed SCoT polymorphisms in the newly synthesized tritordeum lines HT27 and HT22, respectively, and their parents. An analysis of the presence/absence of bands among tritordeums and their parents revealed three types of polymorphic markers: (i) shared by tritordeums and one of their parents, (ii) exclusively amplified in tritordeums, and (iii) exclusively amplified in the parents. No polymorphism was detected among individuals of each parental species. Three SCoT markers were exclusively amplified in tritordeums of lines HT22 and HT27, being considered as polyploidization-induced rearrangements. About 70 % of the SCoT markers of H. chilense origin were not transmitted to the allopolyploids of both lines, and most of the SCoTs scored in the newly synthesized allopolyploids originated from wheat, reinforcing the potential use of tritordeum as an alternative crop.
eng
closedAccess
Tritordeum
Start Codon
Hordeum chilense
Triticum turgidum L. ssp. durum
Targeted (SCoT) markers
Potential of Start Codon Targeted (SCoT) markers for DNA fingerprinting of newly synthesized tritordeums and their respective parents
artículo
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URL
https://digital.csic.es/bitstream/10261/127656/1/accesoRestringido.pdf
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accesoRestringido.pdf.txt
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accesoRestringido.pdf.txt