2024-03-29T04:55:52Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1030522021-12-28T16:49:44Zcom_10261_41com_10261_1com_10261_86col_10261_294col_10261_339
DIGITAL.CSIC
author
Hanke, Tobias
author
Ramiro Ibáñez, M. J.
author
Trigueros, Sonia
author
Roca, Joaquim
author
Larraga, Vicente
2014-10-08T11:52:01Z
2014-10-08T11:52:01Z
2003
Nucleic Acids Research, 2003, Vol. 31 (16) 4917-4928
0305-1048
http://hdl.handle.net/10261/103052
10.1093/nar/gkg671
1362-4962
12907735
We identi®ed a type II topoisomerase enzyme from Leishmania infantum, a parasite protozoon causing disease in humans. This protein, named Li topo II, which displays a variable C-terminal end, is located in the kinetoplast. The cloned gene encoding Li-
TOP2 compensates for the slow growth of topo IIde ®cient mutants of Saccharomyces cerevisiae, resulting in a catalytically active DNA topoisomerasein yeast. Analysis of the speci®c mRNA levels of the Li-TOP2 gene showed variations throughout the
parasite cell cycle in synchronized cells as well as between the distinct forms of the parasite. Thus, the enzyme had higher levels of mRNA expression in the highly infective intracellular form of the parasite, the amastigote, than in the extracellular promastigote form, suggesting a relation with the distinct developmental and infectious phases of the protozoon. In addition, western blot analysis showed
differences in protein expression between the proliferative and non-proliferative forms of L.infantum promastigotes, which displayed similar levels of mRNA. This indicated possible post-transcriptional
regulation mechanisms. The data suggest that Li topo II has a part in DNA decatenation and probably at the initial stages of proliferation in the intracellular form of L.infantum, a parasite that has to
proliferate into the host macrophage to survive its hostile environment in its ®rst moments of intracellular infection.
eng
openAccess
Cloning, functional analysis and post-transcriptional regulation of a type II DNA topoisomerase from Leishmania infantum. A new potential target for anti-parasite drugs
artículo
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