2024-03-28T20:51:12Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/889442021-11-22T12:54:12Zcom_10261_3284com_10261_1col_10261_3285
00925njm 22002777a 4500
dc
Ripoll-Rozada, Jorge
author
Zunzunegui, Sandra
author
Cruz, Fernando de la
author
Arechaga, Ignacio
author
Cabezón, Elena
author
2013
Pilus biogenesis and substrate transport by type IV secretion systems require energy, which is provided by three molecular motors localized at the base of the secretion channel. One of these motors, VirB11, belongs to the superfamily of traffic ATPases, which includes members of the type II secretion system and the type IV pilus and archaeal flagellar assembly apparatus. Here, we report the functional interactions between TrwD, the VirB11 homolog of the conjugative plasmid R388, and TrwK and TrwB, the motors involved in pilus biogenesis and DNA transport, respectively. Although these interactions remained standing upon replacement of the traffic ATPase by a homolog from a phylogenetically related conjugative system, namely, TraG of plasmid pKM101, this homolog could not replace the TrwD function for DNA transfer. This result suggests that VirB11 works as a switch between pilus biogenesis and DNA transport and reinforces a mechanistic model in which VirB11 proteins act as traffic ATPases by regulating both events in type IV secretion systems. © 2013, American Society for Microbiology.
Journal of Bacteriology 195(18): 4195-4201 (2013)
http://hdl.handle.net/10261/88944
10.1128/JB.00437-13
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100006365
23852869
Functional interactions of VirB11 traffic ATPases with VirB4 and VirD4 molecular motors in type IV secretion systems