2024-03-28T09:26:36Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/543312019-07-31T10:39:16Zcom_10261_47com_10261_8col_10261_300
00925njm 22002777a 4500
dc
Mauri, I.
author
Romero Jódar, Alejandro
author
Acerete, L.
author
MacKenzie, Simon
author
Roher, N.
author
Calloi, A.
author
Cano, I.
author
Álvarez, M. C.
author
Tort, Lluis
author
2011
Gilthead seabream (Sparus aurata) and European seabass (Dicentrarchus labrax) were subjected to either
experimental infection with Photobacterium damselae subsp. piscicida or Nodavirus after a period of 2
weeks of crowding in which fish were subjected to a 5-fold increase in density (10e50 kg/m3). Samples
were obtained before the crowding period (0 h or control) and at 24 h and 72 h after crowding from both
groups of infected fish. The Complement haemolytic activity and the expression of the C3 gene were
evaluated in blood and liver samples respectively. The bacteriolytic and lysozyme activities were also
assessed. The results showed that Complement haemolytic activity was reduced at 72 h with both
bacteria and virus in high density Gilthead seabream, and a similar increase was observed at low density.
Bacteriolytic activity under both bacterial and viral challenges for both species was increased at 24 h,
under low density. At high density, the bacterial challenge did not induce significant changes. C3 mRNA
abundance was substantially increased after pathogen treatments in low density groups at 24 h but no
significant changes were detected at high densities.
These results support the idea of the suppressor effect of stressors on the immune system since
a reduction of Complement activity under virus and high density, or lack of response in C3 expression
under high density were observed.
Fish and Shellfish Immunology 30(1): 182-188 (2011)
1050-4648
http://hdl.handle.net/10261/54331
10.1016/j.fsi.2010.10.006
Complement
Density
Stressor
Pathoge
Suppressor
Changes in complement responses in Gilthead seabream (Sparus aurata) and European seabass (Dicentrarchus labrax) under crowding stress, plus viral and bacterial challenges