2024-03-29T01:55:37Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/518402023-01-31T08:44:00Zcom_10261_3284com_10261_1col_10261_3285
00925njm 22002777a 4500
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Fernández López, Raúl
author
Cruz, Fernando de la
author
Casadesús, Josep
author
2005
DNA adenine methylase (Dam-) mutants of Salmonella enterica serovar Typhimurium contain reduced levels of FinP RNA encoded on the virulence plasmid. Dam methylation appears to regulate finP transcription, rather than FinP RNA stability or turnover. The finP promoter includes canonical -10 and -35 modules and depends on the σ70 factor. Regulation of finP transcription by Dam methylation does not require DNA sequences upstream from the -35 module, indicating that Dam acts at the promoter itself or downstream. Unexpectedly, a GATC site overlapping with the -10 module is likewise dispensable for Dam-mediated regulation. These observations indicate that Dam methylation regulates finP transcription indirectly and suggest the involvement of a host factor(s) responsive to the Dam methylation state of the cell. We provide evidence that one such factor is the nucleoid protein H-NS, which acts as a repressor of finP transcription in a Dam- background. H-NS also restrains transcription of the overlapping traJ gene, albeit in a Dam-independent fashion. Hence, the decreased FinP RNA content found in Dam - hosts of S. enterica appears to result from H-NS-mediated repression of finP transcription. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Journal of Bacteriology 187(16): 5691-5699 (2005)
http://hdl.handle.net/10261/51840
10.1128/JB.187.16.5691-5699.2005
16077115
Regulation of finP transcription by DNA adenine methylation in the virulence plasmid of Salmonella enterica