2024-03-28T17:22:29Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/499522020-11-18T13:05:51Zcom_10261_86com_10261_1col_10261_339
00925njm 22002777a 4500
dc
Schvartzman, Jorge Bernardo
author
Martínez-Robles, María Luisa
author
López, Virginia
author
Hernandez, Pablo
author
Krimer, Dora B.
author
2012-03-23
Two-dimensional (2D) agarose gel electrophoresis is one of the most powerful methods to analyze the mass and shape of replication intermediates. It is often use to map replication origins but it is also useful to characterize termination of replication, replication fork barriers and even replication fork reversal. Here, we present protocols, figures and movies with a thorough description of different modes of replication for linear DNA fragments and the corresponding patterns they generate in 2D gels
Methods (2012)
1046-2023
http://hdl.handle.net/10261/49952
10.1016/j.ymeth.2012.03.013
1095-9130)
DNA Replication
Initiation
Termination
Barriers
Fork reversal
2D gels and their third-dimension potential