2024-03-28T15:05:20Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/485412017-04-07T07:26:58Zcom_10261_118com_10261_6col_10261_371
00925njm 22002777a 4500
dc
Reina, Ramsés
author
Bertolotti, Luigi
author
Dei Giudici, Silvia
author
Puggioni, Giantonella
author
Ponti, Nicoletta
author
Profiti, Margherita
author
Patta, Cristiana
author
Rosati, Sergio
author
2010-07-29
The highly divergent SRLV genotype E has recently been characterized in Italy as a low
pathogenic caprine lentivirus in the Roccaverano breed. The availability of a genotype specific diagnostic test based on a comparative assay, using a combination of genotype specific recombinant antigens allows a wide serosurvey in other goat populations. The island of Sardinia still has the highest small ruminant population of any Italian region and crossbreeding has been limited to goats, mainly with the Maltese breed.
A serological survey was carried out on sheep flocks and goat herds, using individual
sera as well as a bulk milk-adapted procedure. Genotype E was identified inmore than 50% of goat herds and none of the sheep flocks thus supporting the idea that this genotype is specifically associated with the goat species. The full-length proviral sequence of a Sardinian isolate revealed and confirmed the deletion of dUTPase subunit and the absence of both vpr gene and the 71 bp repeat of the LTR. Genetic similarity of this isolate with the prototype strain Roccaverano was not more than 84%, supporting the designation of two subtypes within genotype E. Nevertheless, in vitro properties of the Sardinian strain were
different from those of the Roccaverano strain in terms of ability to infect synovial
membrane and produce syncitia. Remarkable differences in the HV1 and HV2 of the env
gene were recorded, with the Sardinian isolate displaying sequence motif more similar to arthritic strains. Data presented suggest diffusion of genotype E is wider than previously thought.
Veterinary Microbiology 144(1-2): 24-31 (2010)
0378-1135
http://hdl.handle.net/10261/48541
10.1016/j.vetmic.2009.12.020
Small ruminant lentivirus
Genotype e
Pathogenic subtype
Sarda goat
Small ruminant lentivirus genotype E is widespread in Sarda goat