2024-03-28T21:41:17Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/369732016-11-14T11:56:06Zcom_10261_75com_10261_6col_10261_328
00925njm 22002777a 4500
dc
Nikoumanesh, Kianoush
author
Ebadi, Ali
author
Zeinalabedini, Mehrshad
author
Gogorcena Aoiz, Yolanda
author
2011-06
In this study, in order to know the variability for a rootstock breeding program genetic diversity and
relationships among 55 Iranian almond genotypes and seven related Prunus species were investigated.
Morphological and molecular analyses were used. Principal component analysis showed that three components
explained 67.6% of the total morphological variation for the first year and 68.06% for the second
year of the study. Leaf traits were predominant in the first component and contributed most of the total
variation. Leaf length and width, as well as, leaf area were highly correlated with each other and correlated
to vigor. Also a negative correlation was found between leaf length/width ratio and vigor. Ward’s
method was used to construct cluster from morphological data which allocated individuals into their
respective species. Out of 100 pre-screened RAPD primers, 16 with reproducible bands and maximum
polymorphism were selected. Two-hundred and sixty bands were scored of which 250 of them were polymorphic.
Average value of polymorphism per primer was 95.81% and maximum value for polymorphism
(100%) was obtained from TIBMBA-14, TIBMBA-17, TIBMBB-05, TIBMBB-08, TIBMBD-09, and TIBMBD-10.
On the other hand, the minimum value was obtained from TIBMBB-16 (86%). Primer TIBMBB-5 gave the
maximum number of bands (25 fragments) and the minimum obtained from TIBMBE-18 (11 fragments).
Genetic similarity based on Jaccard’s coefficient ranged from 0.28 to 0.79 with an average of 0.53. Molecular
analysis revealed a high degree of separation among samples regarding their geographical origin.
Correlation between two approaches was low (R =−0.38). High molecular and morphological variability
indicated that this collection includes rich and valuable plant materials for almond rootstock breeding.
Nikoumanesh K, Ebadi A, Zeinalabedini M, Gogorcena Y. Morphological and molecular variability in some Iranian almond genotypes and related Prunus species and their potentials for rootstock breeding. Scientia Horticulturae 129 (1): 108-118 (2011)
0304-4238
http://hdl.handle.net/10261/36973
10.1016/j.scienta.2011.03.017.
Almonds
Rootstock
RAPD
Vegetative traits
Related Prunus spp.
Morphological and molecular variability in some Iranian almond genotypes and related Prunus species and their potentials for rootstock breeding