2024-03-29T05:54:21Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/323342018-07-23T07:12:11Zcom_10261_54com_10261_1col_10261_307
00925njm 22002777a 4500
dc
Valdés, Francisco
author
Álvarez, Alberto M.
author
Locascio, Annamaria
author
Vega, Sonia
author
Herrera, Blanca
author
Fernández, Margarita
author
Benito, Manuel
author
Nieto, M. Ángela
author
Fabregat, Isabel
author
2002
Fetal rat hepatocytes treated with transforming growth factor β (TGF-β) die by apoptosis. However, a subpopulation of them survives and undergoes an epithelial mesenchymal transition (EMT). This transition also occurs upon incubation with fetal bovine serum. We have isolated the subpopulations that undergo EMT (TGF-β-treated-fetal hepatocytes: TβT-FH; serum-treated-fetal hepatocytes: ST-FH) and show that they present high levels of vimentin and Snail expression and lack cytokeratin 18 and E-cadherin. Both TβT-FH and ST-FH cells require mitogens to grow and maintain the response to TGF-β in terms of growth inhibition. However, they lack differentiation markers such as the liver-enriched transcription factors hepatocyte nuclear factor 4 (HNF-4) or HNF-1α and express the progenitor marker OV-6. Interestingly, the EMT process confers them resistance to the apoptotic effect of TGF-β, with cells showing higher levels of active AKT and Bcl-xL than fetal hepatocytes. In summary, these cells are refractory to the apoptotic effects of TGF-β, showing characteristics of liver progenitors and of some hepatocellular carcinoma cells.
Molecular Cancer Research 1(1): 68-78 (2002)
1541-7786
http://hdl.handle.net/10261/32334
http://dx.doi.org/10.13039/501100004587
http://dx.doi.org/10.13039/100012818
The Epithelial Mesenchymal Transition Confers Resistance to the Apoptotic Effects of Transforming Growth Factor β in Fetal Rat Hepatocytes