2024-03-29T13:15:57Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/237812021-10-27T12:01:40Zcom_10261_22com_10261_1col_10261_275
00925njm 22002777a 4500
dc
Martín-Sanz, Paloma
author
Cascales, Maria
author
Boscá, Lisardo
author
1989-02-01
The sensitivity of 6-phosphofructo-2-kinase to glucagon and cyclic AMP was studied during the perinatal period. In liver homogenates from foetal and neonatal rats, incubation with cyclic AMP produced inactivation of 6-phosphofructo-2-kinase 3 h after birth. The maximal effect was obtained 12 h after birth. In primary cultures of hepatocytes from 22-day-old foetuses, glucogon induced an inhibition of 6-phosphofructo-2-kinase that required 45 min to reach the half-maximal effect. Cycloheximide prevented the glucagon-induced changes in this activity from cultured foetal hepatocytes. These results suggest that the adult form of 6-phosphofructo-2-kinase is rapidly induced after birth, probably by the hormonal changes that occur in this period.
Biochemical Journal 257(3): 795-799 (1989)
0264-6021
http://hdl.handle.net/10261/23781
Glucagon-induced changes in fructose 2,6-bisphosphate and 6-phosphofructo-2-kinase in cultured rat foetal hepatocytes