2024-03-28T14:45:17Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1942132019-11-09T02:07:46Zcom_10261_31891com_10261_2col_10261_31892
00925njm 22002777a 4500
dc
Valdés, Alberto
author
García-Cañas, Virginia
author
Pérez-Sánchez, Almudena
author
Barrajón-Catalán, Enrique
author
Ruiz-Torres, Verónica
author
Artemenko, Konstantin A.
author
Micol, Vicente
author
Bergquist, Jonas
author
Cifuentes, Alejandro
author
2017
The antiproliferative activity of Rosemary (Rosmarinus officinalis) has been widely studied in different in vitro and in vivo models, which demonstrate that rosemary extracts inhibit the cellular proliferation due to its ability to interact with a wide spectrum of molecular targets. However, a comprehensive proteomics study in vivo has not been carried out yet. In the present work, the effects of rosemary extract on xenograft tumor growth has been studied and, for the first time, a shotgun proteomic analysis based on nano-LC-MS/MS together with stable isotope dimethyl labeling (DML) has been applied to investigate the global protein changes in vivo. Our results show that the daily administration of a polyphenol-enriched rosemary extract reduces the progression of colorectal cancer in vivo with the subsequent deregulation of 74 proteins. The bioinformatic analysis of these proteins indicates that the rosemary extract mainly alters the RNA Post-Transcriptional Modification, the Protein Synthesis and the Amino Acid Metabolism functions and suggests the inactivation of the oncogene MYC. These results demonstrate the high utility of the proposed analytical methodology to determine, simultaneously, the expression levels of a large number of protein biomarkers and to generate new hypothesis about the molecular mechanisms of this extract in vivo.
Journal of Chromatography A 1499: 90-100 (2017)
0021-9673
http://hdl.handle.net/10261/194213
10.1016/j.chroma.2017.03.072
1873-3778
http://dx.doi.org/10.13039/501100004587
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100003359
http://dx.doi.org/10.13039/100012818
28389096
Colon cancer
Dimethyl labeling
Mass spectrometry
Rosemary extract
Quantitative proteomics
Xenograft mice
Shotgun proteomic analysis to study the decrease of xenograft tumor growth after rosemary extract treatment