2024-03-29T08:50:20Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1893442021-10-27T12:01:39Zcom_10261_22com_10261_1col_10261_401
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Motiño, Omar
author
Brea, Rocío
author
García-Monzón, Carmelo
author
Vargas, Javier
author
Boscá, Lisardo
author
Francés, Daniel E.
author
Casado, Marta
author
Martín-Sanz, Paloma
author
Agra, Noelia
author
2017
Cyclooxygenase-2 (COX-2) is involved in different liver diseases but little is known about the
significance of COX-2 in the development and progression of fibrosis. In the present work, hepatocytespecific
COX-2 transgenic mice (hCOX-2-Tg) and their wild-type (Wt) littermates were injected with
carbon tetrachloride (CC14) for 9 weeks or subjected to bile duct ligation (BDL) for 21 days to induce liver fibrosis. Different cells types were isolated analyzing the expression of a specific set of rniRNAs implicated in liver fibrosis. Furthermore, considering COX-2 expression in hepatocytes could protect against fibrosis by modulating the activation of hepatic stellate cells (HSC), we evaluated the effect of conditioned medium from isolated hepatocytes of COX-2 in primary HSC cells. Our results provide
evidence that COX-2-dependent PGs protects against fibrosis by decreasing the Jevels of pro-fibrogenic markers a-SMA and collagen l a l and increasing apoptosis of HSC. Furthermore, the constitutive expression of COX-2 in hepatocytes represses rniRNA-23a-5p expression in HSC cells. These results suggest a protective effect exerted by COX-2 in the process of liver fibrosis, possibly through the
downregulation of rniR-23a-5p. Further work is in progress to clarify the role of this miRNA and to identify putative target genes.
EMBO Workshop (2017)
http://hdl.handle.net/10261/189344
Cyclooxygenase-2 expression in hepatocytes protects against liver fibrosis in mice through the regulation of miRNA23a-5p