2024-03-29T06:33:50Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1849152019-11-14T10:29:45Zcom_10261_48com_10261_5col_10261_301
00925njm 22002777a 4500
dc
Emerson, Brent C.
author
Casquet, Juliane
author
López, Heriberto
author
Cardoso, Pedro
author
Borges, Paulo A.V.
author
Oromí, Pedro
author
Strasberg, Dominique
author
Thébaud, Christophe
author
2017-07
Obtaining fundamental biodiversity metrics such as alpha, beta and gamma diversity for arthropods is often complicated by a lack of prior taxonomic information and/or taxonomic expertise, which can result in unreliable morphologically based estimates. We provide a set of standardized ecological and molecular sampling protocols that can be employed by researchers whose taxonomic skills may be limited, and where there may be a lack of robust a priori information regarding the regional pool of species. These protocols combine mass sampling of arthropods, classification of samples into parataxonomic units (PUs) and selective sampling of individuals for mtDNA sequencing to infer biological species. We sampled ten lowland rainforest plots located on the volcanic oceanic island of Réunion (Mascarene archipelago) for spiders, a group with limited taxonomic and distributional data for this region. We classified adults and juveniles into PUs and then demonstrated the reconciliation of these units with presumed biological species using mtDNA sequence data, ecological data and distributional data. Because our species assignment protocol is not reliant upon prior taxonomic information, or taxonomic expertise, it minimizes the problem of the Linnean shortfall to yield diversity estimates that can be directly compared across independent studies. Field sampling can be extended to other arthropod groups and habitats by adapting our field sampling protocol accordingly.
Molecular Ecology Resources 17(4): 694-707 (2017)
1755-098X
http://hdl.handle.net/10261/184915
10.1111/1755-0998.12617
1755-0998
http://dx.doi.org/10.13039/501100007757
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100004336
Taxonomy
Parataxonomy
Spider
Biological species
Beta diversity
A combined field survey and molecular identification protocol for comparing forest arthropod biodiversity across spatial scales