2024-03-19T06:23:47Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1736072022-06-30T11:34:02Zcom_10261_54com_10261_1col_10261_307
00925njm 22002777a 4500
dc
Mihaljević, Bojan
author
Larrañaga, Pedro
author
Benavides-Piccione, Ruth
author
Hill, Sean
author
DeFelipe, Javier
author
Bielza, Concha
author
2018-12-17
[Background] The challenge of classifying cortical interneurons is yet to be solved. Data-driven classification into established morphological types may provide insight and practical values. [Results] We trained models using 217 high-quality morphologies of rat somatosensory neocortex interneurons reconstructed by a single laboratory and pre-classified into eight types. We quantified 103 axonal and dendritic morphometrics, including novel ones that capture features such as arbor orientation, extent in layer one, and dendritic polarity. We trained a one-versus-rest classifier for each type, combining well-known supervised classification algorithms with feature selection and over- and under-sampling. We accurately classified the nest basket, Martinotti, and basket cell types with the Martinotti model outperforming 39 out of 42 leading neuroscientists. We had moderate accuracy for the double bouquet, small and large basket types, and limited accuracy for the chandelier and bitufted types. We characterized the types with interpretable models or with up to ten morphometrics. [Conclusion] Except for large basket, 50 high-quality reconstructions sufficed to learn an accurate model of a type. Improving these models may require quantifying complex arborization patterns and finding correlates of bouton-related features. Our study brings attention to practical aspects important for neuron classification and is readily reproducible, with all code and data available online.
BMC Bioinformatics 19(1): 511 (2018)
http://hdl.handle.net/10261/173607
10.1186/s12859-018-2470-1
1471-2105
http://dx.doi.org/10.13039/100007406
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/100012818
http://dx.doi.org/10.13039/501100010198
30558530
Feature selection
Martinotti
Morphometrics
Feature selection
Martinott
Morphometrics
Towards a supervised classification of neocortical interneuron morphologies