2024-03-28T14:18:39Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/172872018-09-19T12:11:43Zcom_10261_69com_10261_2com_10261_95com_10261_1col_10261_322col_10261_348
00925njm 22002777a 4500
dc
Quirós del Bosque, Ana
author
Ramos González, María Mercedes
author
Muguerza, B.
author
Delgado, M. A.
author
Martín-Álvarez, Pedro J.
author
Aleixandre, Amaya
author
Recio, Isidra
author
2006-12
Among different lactic acid bacteria isolated from raw milk, 4 Enterococcus faecalis strains have stood out as producers of fermented milk with potent antihyperten-sive activity. The peptide ß-casein f(133–138), LHLPLP, was identified as one of the major peptides responsible for the activity of these fermented milk products. A simple method was developed to quantify this peptide in fermented milk using high-performance liquid chromatography coupled in line with mass spectrometry. This procedure does not require any previous sample fractionation or extraction, and direct analysis of the water-soluble extract obtained from the fermented milk can be performed. Validation studies showed sufficient speci-ficity, reproducibility, linearity, and recovery, demonstrating that this method can be used for the routine quantification of LHLPLP during the production of fermented milk products. The developed method was readily applied to quantify the peptide LHLPLP under different fermentation conditions and with different aromatized products.
Journal of Dairy Science 89(12): 4527-4535 (2006)
0022-0302
http://hdl.handle.net/10261/17287
Antihypertensive peptides
Enterococcus faecalis
Quantification
Mass spectrometry
Determination of the antihypertensive peptide LHLPLP in fermented milk by high-performance liquid chromatography-mass spectrometry