2024-03-28T14:18:07Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1664622021-12-27T15:34:37Zcom_10261_74com_10261_6col_10261_327
00925njm 22002777a 4500
dc
Whitehead, Caragh
author
Ostos-Garrido, Francisco J.
author
Reymond, Mathieu
author
Simister, Rachael
author
Distelfeld, Assaf
author
Atienza, Sergio G.
author
Pistón, Fernando
author
Gómez, Leonardo D.
author
McQueen-Mason, Simon J.
author
2018-05
The recalcitrance of secondary plant cell walls to digestion constrains biomass use for the production of sustainable bioproducts and for animal feed.
We screened a population of Brachypodium recombinant inbred lines (RILs) for cell wall digestibility using commercial cellulases and detected a quantitative trait locus (QTL) associated with this trait.
Examination of the chromosomal region associated with this QTL revealed a candidate gene that encodes a putative glycosyl transferase family (GT) 43 protein, orthologue of IRX14 in Arabidopsis, and hence predicted to be involved in the biosynthesis of xylan. Arabinoxylans form the major matrix polysaccharides in cell walls of grasses, such as Brachypodium. The parental lines of the RIL population carry alternative nonsynonymous polymorphisms in the BdGT43A gene, which were inherited in the RIL progeny in a manner compatible with a causative role in the variation in straw digestibility. In order to validate the implied role of our candidate gene in affecting straw digestibility, we used RNA interference to lower the expression levels of the BdGT43A gene in Brachypodium.
The biomass of the silenced lines showed higher digestibility supporting a causative role of the BdGT43A gene, suggesting that it might form a good target for improving straw digestibility in crops.
New Phytologist 218(3): 974-985 (2018)
0028-646X
http://hdl.handle.net/10261/166462
10.1111/nph.15089
1469-8137
http://dx.doi.org/10.13039/501100000268
http://dx.doi.org/10.13039/501100000780
29574807
Biomass
Brachypodium
Gene silencing
GT43
Saccharification
Xylan
A glycosyl transferase family 43 protein involved in xylan biosynthesis is associated with straw digestibility in Brachypodium distachyon