2024-03-29T09:33:57Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/165662022-03-24T14:58:02Zcom_10261_25com_10261_1com_10261_86col_10261_278col_10261_339
00925njm 22002777a 4500
dc
Prieto, Ignacio
author
Kouznetsova, Anna
author
Fütterer, Agnes
author
Trachana, Varvara
author
Leonardo, Esther
author
Alonso Guerrero, Astrid
author
Cano Gamero, Mercedes
author
Pacios-Bras, Cristina
author
Leh, Hervé
author
Buckle, Malcolm
author
Gracía-Gallo, Mónica
author
Kremer, Leonor
author
Serrano, Antonio
author
Roncal, Fernando
author
Albar, Juan Pablo
author
Barbero, José Luis
author
Martínez-Alonso, Carlos
author
Van Wely, Karel H. M.
author
2009-10
Synapsis of homologous chromosomes is a key meiotic event, mediated by a large proteinaceous structure termed the synaptonemal complex. Here we describe a role in meiosis for the murine Death inducer obliterator (Dido) gene. The Dido gene codes for three proteins that recognize trimethylated histone H3 lysine 4 through their aminoterminal PHD domain. DIDO3, the largest of the three isoforms, localizes to the central region of the synaptonemal complex in germ cells. DIDO3 follows the distribution of the central region protein SYCP1 in Sycp3-/- spermatocytes, which lack the axial elements of the synaptonemal complex. This indicates that synapsis is a requirement for DIDO3 incoporation. Interestingly, DIDO3 is missing from the synaptonemal complex in Atm mutant spermatocytes, which form synapses but show persistent trimethylation of histone H3 lysine 4. In order to further address a role of epigenetic modifications in DIDO3 localization, we made a mutant of the Dido gene that produces a truncated DIDO3 protein. This truncated protein, which lacks the histone-binding domain, is incorporated in the synaptonemal complex irrespective of histone trimethylation status. DIDO3 protein truncation in Dido mutant mice causes mild meiotic defects, visible as gaps in the synaptonemal complex, but allows for normal meiotic progression. Our results indicate that histone H3 lysine 4 demethylation modulates DIDO3 localization in meiosis, and suggest epigenetic regulation of the synaptonemal complex.
Chromosoma 118:617-632 (2009)
0009-5915
http://hdl.handle.net/10261/16566
10.1007/s00412-009-0223-7
1432-0886
synaptonemal complex
histones
epigenetics
meiosis
protein localization
protein interaction
Synaptonemal complex assembly and H3K4Me3 demethylation determine DIDO3 localization in meiosis